摘要
目的利用基因芯片技术研究天花粉蛋白对人宫颈癌HeLa细胞基因表达谱的影响,以深入探讨其作用的分子机制。方法提取药物处理前后HeLa细胞总RNA并逆转录成cDNA,在获得的cDNA上分别标记CY3和CY5两种荧光物质,然后与BiostarH-Ⅰ表达谱芯片杂交,扫描后对获得的数据用GenePixPro3·0软件分析。结果两组间存在差异性表达基因78条,其中与细胞凋亡密切相关的NOP56、TN-FSF10、CASP9、DFFB等62条基因表达上调,与细胞黏附及细胞间相互作用相关的COL9A3、MGST3、LGALS3BP等16条基因表达下调。结论天花粉蛋白可以引起HeLa细胞中多个基因的表达改变,基因芯片技术进一步揭示了其诱导HeLa细胞凋亡的作用机制。
Aim To explore effects of TCS on the gene expression profiles of human cervical carcinoma HeLa cells with microarray technique and further investigate its molecular mechanism. Methods RNA from both control and treated HeLa cells were isolated and reversely transcribed to cDNA with the incorporation of cy3 and cy5-labeled dUTP, probes were hybridized with BiostarH-Ⅰ cDNA microarray, chips were scanned and then analyzed by GenePix Pro 3.0 software. Results 78 significantly differently expressed genes were screened out of which up-and down- regulated genes were 62 and 16 respectively, the up-regnlated genes were closely related with apoptosis （such as NOP56,TNFSF10, CASP9, DFFB, etc） and the downregulated genes were associated with the adhesion and interaction between cells （ such as COL9A3, LGALS3BP, MGST3, etc）. Conclusion TCS could result in differential expression of multiple genes in HeLa cells, and DNA microarray technique can provide valuable insight into the molecular mechanism of TCS-induced apoptosis.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第1期99-101,共3页
Chinese Pharmacological Bulletin
基金
湖北省教育厅重大科研资助项目(NoZ200513002)
湖北省自然科学基金计划资助项目(No2002AB141)
