摘要
背景与目的:MCM4是微小染色体维持蛋白家族成员之一,是DNA复制执照因子的成员。本研究检测MCM4在食管癌组织中的表达状态,探讨MCM4与食管癌临床及病理参数的关系。方法:运用RT-PCR方法检测60例食管鳞癌组织、癌旁组织及正常上皮组织中MCM4的表达;探讨MCM4在食管癌组织中的表达与临床及病理参数的关系。结果:MCM4在正常食管上皮中不表达或者弱表达,食管癌旁上皮组织MCM4表达率为33.3%(20/60),食管癌组织中MCM4表达率为65.0%(39/60)。MCM4在食管癌组织中的表达显著高于食管癌旁上皮及正常上皮组织。T3期与T1期相比,MCM4的表达状态差异有显著性。而在不同分级、淋巴结转移、性别和年龄患者中,MCM4的表达差异无显著性。结论:MCM4的表达与食管癌的病理分期有一定的相关性。
BACKGROUND ＆ OBJECTIVE： MCM4 belongs to minichromosome maintenance （MCM） protein family. It is a DNA replication licensing factor. This study was to explore the expression and significance of MCM4 in esophageal squamous cell cancer （ESCC）. METHODS： The expression of MCM4 in 60 specimens of ESCC, paracancer esophageal epithelia, and distant normal esophageal epithelia was detected by reverse transcription-polymerase chain reaction （RT-PCR）. The Correlations of MCM4 expression to clinicopathologic features of the 60 ESCC patients were analyzed. RESULTS： The positive rate of MCM4 was significantly higher in ESCC than in paracancer esophageal epithelia （65.0% vs. 33.3%, P〈 0.001）; MCM4 was not expressed or weakly expressed in distant normal esophageal epithelia. The positive rate of MCM4 was significantly higher in stage T3 ESCC than in stage T1 ESCC （73.7% vs. 25.0%, P〈0.05）. The expression of MCM4 had no correlations to grade, lymph node metastasis, sex, and age. CONCLUSION： The expression of MCM4 might be related to the pathologic stage of ESCC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2007年第1期96-99,共4页
Chinese Journal of Cancer
基金
国家自然科学基金(No.30572103)
广东省自然科学基金(No.5300808)
中国博士后科学基金(No.2005037612)~~
