摘要
用钳位均匀电场脉冲电泳(CHEF)系统分析了克鲁斯假丝酵母(Candida krusei),郎比可假丝酵母(C. lambica)和粗状假丝酵母(C. valiad)的模式菌株的电泳核型,发现这三种表型相似的假丝酵母却具有互不相同的染色体DNA分子带型,为其分类学研究提供了可靠的鉴别依据。在常规分类学研究的基础上,测定了AS 2.75(原定种名为(C. incospicua),AS2.1182(原定种名为 C. lambica)和AS 2.1772(未定种)等三株假丝酵母的G+C含量和脉冲电泳核型。通过对已报道的C. inconspicua的G+C含量及上述三种假丝酵母模式菌株的脉冲电泳核型的比较分析证明,AS 2.75和AS 2.1772为粗状假丝酵母(C. valida),AS 2.1182为克鲁斯假丝酵母(C. krusei)。
The electrophoretic karyotypes of the type strains of Candida krusei(CBS 573), C. lambica (CBS 1876) , and C. valida(CBS 638) were examined by using the CHEF (contour-clamped homogenous electric field) system for pulsed field gel electrophoresis (PFGE). The chromosomal DNA banding patterns of the three phenotypically similar taxa were shown to be clearly different. Among the Candida strains preserved in China General Microbiological Culture Collection Center (CGMCC), two strains, AS 2.75 and AS 2.1182, were originally identified as C. inconspicua and C. lambica, respectively. However, on the basis of morphological and physiological recharacterization and G+C content determination, AS 2.75 with 44.4 mol% G+C and AS 2.1182 with 41.5 mol% G+C were reidentified as C. valida and C. krusei, respectively. The reidentification of them was confirmed by comparative electrophoretic karyotyping of the two strains and the type strains of C. krusei, C. lambica, and C. valida, which indicates that a ) AS 2.75 and CBS 638 have similar chromosomal DNA banding patterns consisted of two to three bands ranging in size from 2.1 to 3.1mb; b) AS 2.1182 and CBS 573 have almost identical electrophoretic karyotypes composed of two bands with molecular sizes ranging from 1.6 to 3.2 mb; and c) CBS 1976 has an unique karyotype with at least five chromosomal DNA-bands ranging in size from 1.7 to 3.7 mb. A formerly unidentified strain AS 2.1772 was likewise classified as C. valida based on its morphological and physiological properties, its G+C content, and its electrophoretic karyotype.
出处
《微生物学报》
CAS
CSCD
北大核心
1996年第5期329-334,共6页
Acta Microbiologica Sinica
关键词
假丝酵母属
分类
脉冲电泳核型
G+C content, Electrophoretic karyotype, Candida krusei, Classification
