摘要
目的:观察孔隙率不同的锶磷灰石多孔陶瓷的骨融合能力差异。方法:实验于2004-06/2005-02在上海第九人民医院动物房和动物手术室完成。24只健康新西兰大白兔随机分为4组,在双侧前肢制造10mm桡骨缺损,分别植入孔隙率为50%、60%、70%的锶磷灰石多孔陶瓷(掺锶5%克分子数的羟磷灰石)作为实验组,以植入70%孔隙率的羟磷灰石多孔陶瓷作对照组。在术后3,6,12,24周处死动物,大体及X射线摄片观察新骨生成情况,取缺损两端各3mm长骨组织作组织学图像观察,24周标本作四环素荧光标记及不脱钙硬组织图像观察。结果:24只白兔中有4只死亡,后补充,进入结果分析24只。①术后3周,70%孔隙率锶磷灰石和70%孔隙率羟磷灰石多孔陶瓷的孔隙内有少量骨基质及成骨细胞,6周,70%孔隙率的锶磷灰石多孔陶瓷的成骨现象最明显,其余,70%孔隙率羟磷灰石>60%孔隙率锶磷灰石>50%孔隙率锶磷灰石。②24周荧光显示70%孔隙率锶磷灰石的荧光强度和面积最丰富。③24周硬组织图像显示锶磷灰石陶瓷随孔隙率的增高,陶瓷的降解现象明显。结论:同一孔径、孔隙率高的锶磷灰石成骨现象早,骨量丰富,24周材料降解较明显;同一孔径、同一孔隙率条件下,锶磷灰石比羟磷灰石成骨量大,生物降解明显。
AIM： To evaluated the influence of different total （porosity） of strontium substituted hydroxyapatite （Sr-HA） on their bone recolonization ability. porous volumes porous ceramics METHODS： The experiment was carried out in the Animal Chamber and Animal Laboratory, Shanghai Ninth People＇s Hospital from June 2004 to February 2005. Totally 24 healthy New Zealand rabbits were divided into four groups at random, which were implanted with total porous volumes as 50%, 60%, 70% Sr-HA （mixed with HA in 5% mole） and 70% HA respectively in bilateral 10-mm defects produced at medial radius bone of rabbits. The interface between bone and ceramic samples were observed by X-ray, and histological imaging was analyzed to evaluate the bone ossification in each 3 mm of defects at 3, 6, 12 and 24 weeks post-operation. Tetracycline fluorescent marker and un-decalcified image were made in the samples at 24 weeks post-operation. RESULTS： Totally 24 rabbits were involved in the result analysis after complements for 4 deaths.①At 3 weeks post-operation, there were a few new bone matrix and osteoblasts appeared in 70% Sr-HA and 70% HA. At 6 weeks post-operation, the most abundant new bone of four porous ceramics came out in 70% Sr-HA, as for others, 50% Sr-HA 〈 60% Sr-HA 〈 70% HA.②At 24 weeks post-operation, the brightest and widest fluorescence was present in 70% Sr-HA ceramics. ③At 24 weeks post- operation, the biodegradation of Sr-HA ceramics became more obvious when the total porous volumes became larger. CONCLUSION： Sr-HA of the same pore size and higher porous volumes can form new bone earlier and more, and have obvious biodegradation. When at the same pore size and porous volumes of ceramics, Sr-HA can create more new bone and more obvious biodegradation than HA.
出处
《中国临床康复》
CSCD
北大核心
2006年第45期57-59,I0006,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助(59872020
30470780)~~
