摘要
为了考察大孔吸附树脂对苦参水提液中氧化苦参碱的分离效果，选择5种大孔吸附树脂，并采用HPLC定量分析法，研究了不同大孔吸附树脂对氧化苦参碱的吸附性能。结果表明，大孔吸附树脂LSA-21和LSA-30的分离效果明显优于LSA-40，HP-10和AB-8;LSA-21在pH10．0和0℃时吸附效果最好，其动态饱和吸附率为14．52mg／mL，静态饱和吸附率为6．88mg／mL。最适洗脱剂为体积分数30％乙醇，解吸率可达90％以上，由此得到的氧化苦参碱含量约为37％。静态饱和吸附率随温度的升高而降低，其中在较低温度范围（0～10℃）内影响显著；静态吸附速率随时间的延长而变小，其中在吸附的前2h内。吸附速率很大且基本恒定，2h之后吸附速率变得非常小。
The separation effects of oxymatrine from Sophora flavescens with five types of macroporous absorption resins were studied. The results showed that macroporous absorption resin LSA-21 and LSA-30 were much better than LSA-40,HP-10 and AB-8 in absorption ratio;The optimum absorption conditions of LSA-21 were pH 10.0 and 0℃,and its maximum dynamic absorption ratio and maximum static absorption ratio were 14.52 mg/mL and 6.88 mg/mL respectively,ideal eluent was 30% ethanol,which led to 〉190% debsorption ratio and about 37% oxymatrine in dry eluate;The static absorption ratio would drop as the temperature rose and was more obvious in lower temperature range（0-10℃）,and meanwhile the static absorption speed slowed with the prolonging of time ,the absorption ratio being fast and constant in the first 2 hours,then much slower.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2006年第11期129-132,共4页
Journal of Northwest A&F University(Natural Science Edition)
关键词
苦参
氧化苦参碱
大孔吸附树脂
吸附
分离
Sophora flavescens
oxymatrine
macroporous absorption resin
absorption
separation
