摘要
目的研究胃癌和十二指肠溃疡患者中的肿瘤坏死因子（TNF）-β基因多态性，并分析TNF-β基因多态性与幽门螺杆菌（H.pylori）感染相互作用与胃癌和十二指肠溃疡的关系。方法采用PCR-RFLP方法对124例胃癌、83例十二指肠溃疡及96例健康对照者进行基因多态性分析。用ELISA方法检测血清Hp抗体。结果胃癌组TNF-β＋252＊G基因频率明显低于对照组（51．2％对63．0％，P＜0．05），TNF-β＋252＊A基因频率明显高于对照组（48．8％对37．0％，P＜0．05），TNF-β＋252A／A基因型的优势比值为2．44（95％CI 1．18—5．03）。在血清H．pylori抗体阳性的胃癌组中，这种基因多态性的影响表现得更明显，优势比值为2．52。十二指肠溃疡和对照组基因频率比较差异无统计学意义（P＞0．05），十二指肠溃疡组的血清H.pylori抗体阳性率明显高于健康对照组（P＜0．01）。结论中国汉族胃癌病人与TNF-β基因多态性相关联，TNF-β＋252A／A基因型与胃癌的易感性有关；十二指肠溃疡病人与TNF-β基因多态性无关联。H．pylori感染是十二指肠溃疡的独立危险因素。
Objective To study tumor necrosis factor（TNF）-β gene polymorphism gastric cancer and analyze the interaction of TNF-β gene polymorphism and H. pylori infection in gastric cancer and duodenal ulcer. Methods One hundred and twenty four gastric cancer patients, 83 duodenal ulcer patients and 96 control subjects were enrolled. TNF-β gene polymorphism was analyzed with polymerase chain reaction-restriction fragment length polymorphism（ PCR-RFLP）. Serum H. pylori IgG was tested by ELISA. Results The gene frequency of allele A of TNF-β ＋ 252 in gastric cancer patients was significantly higher than that in control subjects（48.8 % vs37.0 %, P 〈 0.05）, but the gene frequency of allele G was significantly lower than that in control subjects（51.2%vs63.0%, P 〈 0.05） .OR of the TNF-β ＋ 252A/A homozygote was 2.44（95 % CI 1.18 - 5.03）. With H. pylori seropositivity, the OR estimate was higher. There were no significant differences in the gene frequencies of allele A, G between duodenal ulcer and control subjects（ P 〉 0.05）. H. pylori seropesitive rate in duodenal ulcer patients were prominent than that in control subjects（ P 〈 0.01 ）. Conclusion TNF-β gene polymorphism is a useful marker for gastric cancer,in Chinese. TNF-β ＋ 252A/A is a susceptive marker for gastric cancer. H. pylori infection is an independent risk of duodenal ulcer.
出处
《胃肠病学和肝病学杂志》
CAS
2006年第6期581-583,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
福建省科技厅重点科研项目资助
2003Y015
