摘要
目的:筛选肾病综合征患者热休克蛋白90β(heatshockprotein90,HSP90)基因突变,并分析该突变与糖皮质激素治疗敏感性的关系.方法:提取300例经糖皮质激素治疗的肾病综合征患者血液DNA,采用聚合酶链反应(polymerasechainreaction)和自动荧光测序方法,对HSP90基因intron9,exon10,intron10,exon11,3非编码区域DNA序列进行突变分析,结合临床资料探讨突变与糖皮质激素治疗敏感性的关系.结果:在肾病综合征患者中发现了2个HSP90β基因点突变,分别为intron10的7489C>T和3非编码区的7612C>T,其发生频率在糖皮质激素敏感和不敏感患者中分别为(2.44%,3.68%)和(2.44%,2.94%),两组患者的突变频率无统计学差异(P>0.5).结论:在肾病综合征患者中发现9例7489C>T和8例7612C>T突变,在敏感和不敏感组的发生频率无显著差异,提示HSP90β表达量与GC治疗敏感性无显著相关.
AIM： To screen the mutation of heat shock protein 90β（HSP90β） gene in patients with primary nephrotic syndrome and to investigate the association between the mutation and the sensitivity to glucocorticoid therapy. METHODS： Extract the blood DNA of the 300 glucocoiticoid-treated primary nephrotic syndrome patients. PCR approach was used to amplify intron 9, exon10, intron 10, exonll and 3＇- untranslated region of HsP90β, and automated fluorescent sequencing technology was applied to detect the mutation of the region. RESULTS： Two point mutations of HsP90β, 7489 C 〉 T in intron 10 and 7612 C 〉 T in 3＇- untranslated region were found in primary nephrotic syndrome patients. The mutation frequency of glucocoiticoid-sensitive and glucocoiticoid-resisted patients were 2.44%, 3.68% and 2.44% , 2.94% ; there was no significant difference in the 2 groups （ P 〉 0.5 ）. CONCLUSION ： This study has found 9 cases of 7489 C 〉 T and 8 cases of 7612 C 〉 T in primary nephrotic syndrome patients ,but there was no significantly difference in the occurrence frequency between glucocoiticoid-sensitive and glucocoiticoid-resisted groups. The expression quantity of HSP90β has no relation to glucocoiticoid therapeutic effect.
出处
《第四军医大学学报》
北大核心
2006年第24期2300-2302,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金项目(30470988)
