摘要
目的通过β-氨基丙腈(beta-aminopropionitrile,BAPN)来研究赖氨酰氧化酶(Lysyl Oxidase,LOX)对体外培养的人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)血管生成的影响,并对其机制进行初步探讨。方法Northern Blot分析人脐静脉内皮细胞中LOX表达,纤维蛋白凝胶体外血管生成检测试剂盒检测血管内皮细胞生长因子和LOX抑制剂BAPN对人脐静脉内皮细胞血管生成的作用,Western Blot检测BAPN处理对MAPK、Akt和PLCγ1的影响。结果人脐静脉内皮细胞中有LOX表达;血管内皮细胞生长因子可促进血管生成,而BAPN可拮抗这种促进作用;BAPN处理后的人脐静脉内皮细胞磷酸化的MAKP表达减少。结论β-氨基丙腈可抑制由血管内皮细胞生长因子诱导的血管生成,提示LOX可能对血管生成有促进作用,其机制是影响信号传导通路中磷酸化的MAKP表达。
Objective To investigate the effect of Lysyl Oxidase （LOX） on human umbilical vein endothelial cell （HUVEC） angiogenesis by beta-aminoproprionitrile （BAPN）. Methods Expression of LOX in HUVEC was detected by Northern blot analyses. Angiogenesis were tested using CHEMICON fibrin gel in vitro angiogenesis assay kit and HUVEC was treated with vascular endothelial growth factor （VEGF） and 100- 400μM BAPN, a specific inhibitor of LOX. With western blot, the level of phospho - MAPK,Akt and PLCγ1 in HUVEC and BAPN pre - treated HUVEC were determined. Results LOX was found in HUVEC. With the supplement of VEGF, the number of newly formed vessel branching point increased compared to cells without VEGF supplements. BAPN reduced the branching points back to control level. Western blot analysis revealed that BAPN down-regulated phospho-MAPK in HUVEC. Conclusion BAPN suppresses HUVEC angiogenesis. It suggests that LOX may promote angiogenesis through up-regulating phospho - MAPK.
出处
《宁夏医学院学报》
2006年第6期469-471,481,共4页
Journal of Ningxia Medical College
基金
教育部科技研究重点项目(编号205176)
宁夏国际科技合作计划项目(2005-86号)
关键词
β-氨基丙腈
赖氨酰氧化酶
血管生成
人脐静脉内皮细胞
beta-aminoproprionitrile
lysyl oxidase
angiogenesis
human umbilical vein endothelial cell
