摘要
目的检测第Ⅷ因子相关抗原（FⅧ-RAg）和血管内皮生长因子（VEGF）在翼状胬肉中的表达及微血管密度（MVD）,探讨其在翼状胬肉发生发展中的作用。方法应用免疫组化Elivision法检测20例翼状胬肉、10例正常结膜中FⅧ-RAg和VEGF的表达,并检测MVD。结果20例翼状胬肉和正常结膜中VEGF的阳性表达率分别为90%,20%。VEGF阳性表达两组间差异有统计学意义P〈0.01。FⅧ-RAg的阳性表达率分别为80%、30%,FⅧ-RAg阳性表达两组间差异有统计学意义,P〈0.05,翼状胬肉和正常结膜中微血管密度（MVD）分别为45.85±11.06、23.64±6.87,两组间差异有统计学意义,P〈0.001。结论VEGF、FⅧ-RAg在翼状胬肉中的高表达,MVD升高,提示有新生血管化的发生,可能与翼状胬肉的发生、发展和术后复发有关。
Objective To investigate the role of expression of vascular endothelial growth factor （VEGF） and Factor VⅢ related antigen（FVllI-RAg） and measurement of microvascular density（MVD） in the occurrence and development of human pterygium. Methods The immunohistochemical Elivision method was used to examine the expressions of F Ⅷ -RAg and VEGF in 20 cases of pterygium and 10 cases normal conjunctiva,MVD was measured as well. Results The positive rate of VEGF was 90.0% and 20% and F Ⅷ -RAg was 80% and 30%,respectively in pterygium and in the normal conjunctiva. There was significant difference in the expressions of F Ⅷ -RAg and VEGF and microvascular density between the pterygium and normal conjunctiva,P〈0.05 and P〈0.001. Conclusions There is a high F Ⅷ -RAg and VEGF expressions in pterygium which suggesting that there is abnormal blood vessel multiply in pterygium,that the F Ⅷ -RAg and VEGF plays an important role in the incidence and development of pterygium and that specific FⅧ -RAg and VEGF inhibitorbecome a new target for the therapy of pterygium.
出处
《中国实用眼科杂志》
CSCD
北大核心
2007年第11期1264-1266,共3页
Chinese Journal of Practical Ophthalmology
关键词
FⅧ相关抗原
血管内皮生长因子
翼状胬肉
血管形成
免疫组织化学
Factor Ⅷ related antigen
Vascular endothelial growth Factor
pterygium
Angiogenesis
Immunohistochemistry
