摘要
目的探讨褪黑素（MT）对于高糖诱导的肾小球系膜细胞（MC）凋亡中的作用及机制。方法光学显微镜观察大鼠MC凋亡的形态学变化；流式AnnexinV／PI双染法检测不同浓度MT对大鼠MC细胞凋亡率的影响；免疫细胞化学检测细胞色素C的表达改变。结果MT对30mmol／L葡萄糖诱导的MC高凋亡率有明显的抑制作用，呈剂量依赖性关系；并能明显抑制细胞色素C的表达。结论MT可抑制高糖诱导的大鼠MC高凋亡率，这种作用部分通过调控细胞色素C来实现的。
Objective To investigate the effects and mechanisms of Melatonin on the apoptosis of rat mesangial cells （MC） induced by high glucose. Method Rat mesangial cells were cultured in DMEM with high glucose concentration （30 retool/L） and different concentrations of Melatonin（MT- low dose group; MT-mild moderate dose group; MT-high dose group） for 72 h. The apoptosis ratio was tested by flow cytometry AnnexinV/PI double stains. The expression of cytochrome C was tested by immunohistochemical method. Results Exposed to high glucose（30 mmol/L） for 72 h, as compared to control（30. 47± 2. 59%） ,the apoptosis ratio of MT-low dose group were 24. 43% ± 1.70% ; MT-mild moderate dose group were 20, 07% ± 1.93%; MT-high dose group were 16. 32%± 1.41%, fewer MCs underwent apoptosis（P〈0. 01 ） in MT group. It showed a dose-dependent manner. As compared to control, the expression of cytochrome C in mesangial ceils were significantly decreased in MT group. Conclusions Melatonin can inhibit the MCs apoptosis induced by high glucose（30mmol/ L）, which partly attributed to lowering the expression of cytochrome C.
出处
《临床肾脏病杂志》
2006年第6期268-270,F0003,共4页
Journal Of Clinical Nephrology
