摘要
短毛五加总皂苷在体外抑制人血小板聚集和血小板因子４释放１陈小川，夏兰２，胡松３，黄光（海南医学院药理教研室，海口５７０１０２；２海南省人民医院血液室，海口５７００１１；３海南心血管病医院，海口５７０２０８，中国）关键词短毛五加；皂苷类；血小板聚集；血...
IM: To study the effects of Acanthopanax
gracilistylus var pubescens Li saponins (AGVPS) on human
platelet aggregation and platelet factor 4 (PF4) liberation in
vitro . METHODS: Human platelet aggregations induced by ADP,
adrenaline, and collagen were measured turbidimetrically. The
aggregation curve was recorded on a platelet aggregometer and the
maximal aggregation rate (AR max ), effective deaggregation rate
in 5 min (DR 5 min ) and lag time (LT) were autocalculated by
the built in microcomputer; PF4 liberation from human platelets
stimulated by ADP and collagen was determined by recording the
heparin thrombin clotting time (HTCT). Thrombosis was tested by
weighing the wet and dry thrombi formed in a siliconized revolving
ring. RESULTS: AGVPS inhibited in vitro the AR max with IC
50 of 1 33 (95 % confidence limits: 1 09-1 63, ADP induced), 1
66 (1 54-1 79, adrenaline induced), and 4 2 g·L -1 (0
6-29, collagen induced). The DR 5 min (on ADP induced
aggregation) and LT (collagen induced) were also increased as well.
Meanwhile, AGVPS 0 63-2 50 g·L -1 prolonged HTCT on ADP
and collagen stimulated PF4 liberation. At 0 34-1 39 g·L -1 ,
AGVPS reduced the wet and dry weight of thrombi formed in vitro .
CONCLUSION: AGVPS inhibits human platelet aggregation, liberation,
and thrombosis in vitro , suggesting its possible antithrombotic
action in man.
出处
《中国药理学报》
SCIE
CAS
CSCD
1996年第6期523-526,共4页
Acta Pharmacologica Sinica
关键词
短毛五加
皂苷类
血小板聚集
血小板因子4
Acanthopanax gracilistylus
saponins
platelet
aggregation
platelet factor 4
thrombosis
