摘要
[目的]探讨神经生长因子(nerve growth factor,NGF)和脑源性神经生长因子(brain derived nervegrowth factor,BDNF)基因修饰的骨髓基质细胞(bone m arrow stem cells,BMSCs)静脉移植治疗脊髓损伤。[方法]SD大鼠制备成脊髓损伤动物模型。随机分为损伤对照组(A组)、BMSCs静脉移植组(B组)、NGF、BDNF基因修饰的BMSCs静脉移植组(C组)、正常对照组(D组)。治疗后2、6、10周,每组动物分别进行联合行为评分(GBS)、运动诱发电位(MEP)、感觉诱发电位(SEP)检查、双下肢功能测定(爬坡试验),评价脊髓损伤功能恢复情况。[结果]随时间的延长,C组GBS、MEP、SEP及下肢功能明显改善。与其他组相比较,差异有显著性,P<0.05。[结论]NGF、BDNF基因修饰的BMSCs静脉移植能部分恢复损伤脊髓的功能。
[ Objective ] To study NGF, BDNF gene modified bone marrowe stem cells transplantation with vein on spinal cord injury. [ Method] Spinal cord injured rats were divided in to 3 groups. Group C consisted of the rats treated with the transplantation of modified NGF,BDNF genes BMSCs;group B of the rats with transplantation of BMSCs without genetic modification;group A of the rats without treatment and group D was the normal contral. At 2,6,10 weeks the rats were examined with GBS score and neurological function were tested. [ Result] The vein with tintracord transplantation of modified BMSCs group was improved by the GBS score and neurological outcome of injured spinal cord was achieved well. [ Conclusion] The vein with transplantation of modified NGF, BDNF genes BMSCs can recover of spinal cord injury.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第24期1900-1902,共3页
Orthopedic Journal of China
关键词
基因修饰
骨髓基质细胞
神经营养因子
静脉移植
脊髓损伤
genetic modification
bone marrow stem cells
transplantation with vein
spinal cord injury
