摘要
目的观察高功率微波(HPM)辐射后大鼠海马N-甲基-D-天冬氨酸(NMDA)受体表达的变化及其意义。方法采用10 mW/cm^2和100 mW/cm^2 HPM辐射110只Wistar雄性大鼠,于辐射后6 h、1 d、7 d、14 d和28 d活杀取海马组织,采用免疫组化、原位杂交和图像分析等技术分析海马组织中NMDA受体——NR1、NR2A和NR2B的蛋白及mRNA含量的变化。结果10 mW/cm^2及100 mW/cm^2 HPM辐射后,可见大鼠海马神经元固缩等病理变化,100 mW/cm^2组的病变较10 mW/cm^2组严重,且恢复迟。2个辐射组的NR1、NR2A及NR2B的表达均增强；10 mW/cm^2组辐射后6 h,NMDA受体表达始见增加,1 d达高峰,28 d基本恢复；100 mW/cm^2组照后6 h,NMDA受体表达始见增加,7 d达高峰,28 d基本恢复,且与10 mW/cm^2组相比,100 mW/cm^2组增高明显,恢复较迟。NR1mRNA变化规律与其蛋白表达类似。结论10 mW/cm^2及100 mW/cm^2 HPM辐射可造成大鼠海马神经元损伤,使NMDA受体表达上调,参与HPM致海马组织损伤的病理生理过程。
Objective To investigate the expression of n-methyl d-asparate receptor（NMDAR） in the hippocampi of rats after high power microwave radiation（HPMR）. Methods One hundred and ten healthy Wistar male rats were irradiated with HPMR at a mean power density of 10 mW/cm^2 or 100 mW/cm^2 , then sacrificed at 6 h, 1 d, 7 d, 14 d and 28 d after irradiation. The expression of NMDA receptor protein and its mRNA were measured by using immunohistochemistry, in-situ hybridization and image analysis. Results Karyopyknosis and other pathological changes were observed, and these were more severe after 100 mW/cm^2 radiation than after 10 mW/cm^2 radiation. The expression levels of receptor proteins NR1 , NR2A and NR2B were increased in both groups, beginning at 6 h, peaking at 1 to 7 days, and recovering by the 28th day. The expression of NR1 mRNA was similar to that of NR1 protein. Conclusion Injuries to hippocampal neurons and altered NMDAR expression result from HPMR at either 10 mW/cm^2 or 100 mW/cm^2. NMDAR may contribute to the pathology of radiation injuries to hippocampal tissues.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第12期800-803,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
全军"十五"杰出中青年科研基金(No.04J016)
