摘要
目的探讨中药生血合剂对慢性再生障碍性贫血(简称再障)患者外周血T细胞表型变化的干预作用。方法将40例患者随机分为生血合剂(SXM)组和环孢菌素(CsA)组,各20例;另设正常组10例。用流式细胞仪检测正常组及患者治疗前后外周血T细胞表面抗原CD4+、CD8+、T细胞受体(αβTCR+、γδTCR+)表达水平。结果再障患者CD4+表达和CD4+/CD8+比值明显低于正常组(P<0.01,P<0.001),CD8+、γδTCR+表达明显高于正常组(P<0.01,P<0.001),αβTCR+表达与正常组无明显差异(P>0.05)。治疗后两组CD4+、CD4+/CD8+比值均增高,CD8、γδTCR+下降(P<0.05,P<0.01,P<0.001),组间比较无显著差异(P>0.05)。结论再障存在CD4+表达降低和CD8+、γδTCR+表达增高的细胞表型变化,生血合剂可干预上述T细胞介导的细胞免疫紊乱,具有与免疫抑制剂CsA相当的作用。
Objective To explore the intervention of ＂Shengxue Mixture＂ on T lymphocyte phenotype in chronic aplastic anemia（AA） patients, Methods Detect the peripheral blood T lymphocyte surface antigen CD4^＋.CD8^＋ and T cell receptor（αβ TCR. γβ TCR） of 40 chronic AA patients before and ＇after treatment with this Mixture and Cyclosporin A （CsA） by the flow cytometer, Results In chronic AA patients,the CD4^＋ expression and the ratio of CD4^＋/CD8^＋ was significantly lower than normal and this Mixture increase them;the CD8^＋ and γβTCR expression was significantly higher than normal and the Mixture could decrease them; the αβ TCR expression had no significant difference with normal one,there was no statistical significance between the Mixture group and CsA control group. Conclusion There are changes of T lymphocyte phenotype about decreasing CD4^＋ expression and increasing CD8^＋,γβTCR expression in AA. This Mixture can regulate immune disorder,with the similar effects to immune-suppressive dmgs.
出处
《上海中医药杂志》
北大核心
2006年第12期18-19,共2页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市教育委员会资助项目(01703)
