摘要
用体外液体培养法，观察组胺Ｈ＿２受体激动剂４－ＭＮ对人类早幼粒白血病细胞系ＨＬ＿（６０）细胞的增殖和分化的影响。结果表明，１０￣（－３）～１０￣（－５）ｍｏｌ·Ｌ￣（－１）的４－ＭＨ能抑制ＨＬ＿（６０）细胞增殖，促进其分化。用胞浆ＲＮＡ斑点杂交技术检测到１０￣（－４）ｍｏｌ·Ｌ￣（－４）４－ＭＨ能降低ＨＬ＿（６０）细胞ｃ－ｍｙｃ基因的ｍＲＮＡ表达。
he effects of 4-MH(4-methylhistamine,an agonist of histamine H2-receptor)on the prolif-eration and differentiation of the HL60 promyelocytic leukemia cell line were investigated in theliquid cuIture in vitro.It showed that 4-MH at concentrations ranging from 10-5mol·L-1 to 10-3mol·L-1 significantly inhibited the proliferation and induced the differentiation of HL60 cells(P<0.05),The dot blot technique of total cellular RNA with α-32 P nicktranslated c-myc gene asprobe was used to study the changes in transcription level of c-myc oncogene as HL60 leukemiccells were treated bv 4-MH.The results showed that 4-MH 10-4 mol·L-1 could decrease level ofc-myc mRNA and inhibit the transcription of c-myc mRNA in HL60 cells.
出处
《湖南医科大学学报》
CSCD
1996年第5期375-377,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
甲基组胺
组胺
激动剂
HL60细胞
C-MYC基因
histamine
agonist
proliferative suppression
induce differentiation
oncogene
HL60
