摘要
在ＨＬ－６０白血病细胞的体外培养中，组胺Ｈ＿２受体激动剂４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有轻微的抑制作用，而组胺Ｈ＿２受体拮抗剂雷尼替丁（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有较强的抑制作用。但用１０￣（－６）ｍｏｌ／Ｌ的雷尼替丁预处理ＨＬ－６０细胞则可部分地拮抗４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０白血病细胞的抑制作用。
The results obtained from liquid suspension culture,semisolid culture and ￣3H-TdR incorpo-ration into HL-60 leukemia cells indicated that histamine H_2 receptor agonist 4-methylhistamine(4-MH) had slight suppressive effect on HL-60 leukemia cells when the 4-MHdoses were 10￣(-8)-10￣(-4)mol / L.The IC_50 of 4-MH in HL-60 colony culture was 1.74 ×10￣(-5)mol / L. Histamine H_2 receptor antagonist ranitidine exerted its suppressive effect onHL-60 leukemia cells when ranitidine 10￣(-8)mol / L to 10￣(-4)mol / L were added.The IC_50 ofranitidine in HL-60 colony culture was 4.24×10￣(-8)mol / L.Therefore, histamine H_2 receptor an-tagonist ranitidine exerted greater suppressive effect on HL-60 leukemia cells than histamine H_2receptor agonist 4-MH did.When HL-60 leukemia cells were pretreated with ranitidine10￣(-6)mol / L for 0.5h,which had no supressive effect on HL-60 cells,the inhibitory effect of4-MH(10￣(-8)-10￣(-4)mol / L) on HL-60 leukemia cells could be antagonized to some extent.Thesephenomena suggest that histamine H_2 receptor agonist 4-MH and its antagonist ranitidine mayexert their suppressive effects on HL-60 leukemia cells through different mechanisms whichshould be clarified in further experiments.
出处
《中国应用生理学杂志》
CSCD
1995年第4期294-298,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助课题
