摘要
以尼龙网作载体，用戊二醛交联将葡萄糖氧化酶（ＧＯＤ）固定装成酶柱并联结于流动体系中，采用流动注射分光光度法研究磁场对固定化ＧＯＤ催化活性的影响。结果表明，在０℃、５℃、４５℃下以１．０×１０－２ｍｏｌ·Ｌ－１的葡萄糖为底物及５５℃下以０．１ｍｏｌ·Ｌ－１的葡萄糖为底物时，０．２２Ｔ静磁场能显著提高固定化ＧＯＤ的反应速度，且磁场效应与底物浓度及磁场作用时间有关。用动力学方法考察了不同强度的磁场对溶解ＧＯＤ反应进程的影响，未发现磁场效应。
Glucose oxidase was covalently bound via glutaraldehyde onto nylon net and packed into a column in flow system. The effects of magnetic field on enzymic reaction of immobilized and dissolved glucose oxidase have been studied by flow injection spectrophotometry.The results show that the rates of immobilized glucose oxidase catalyzed reactions remarkably increase when the reacions were carred out at 0℃,5℃,and 45℃ with a glucose concentration of 1.0×10 -2 mol·L -1 ,as well as at 55℃ with a glucose concentration of 0.1 mol·L -1 .The effects of magnetic field depend on the concentration of substrate and exposure time. In addition, the effect of magnetic field on process of dissolved glucose oxidase catalyzed reaction has also been investigated by kinetic method,but no effects observed. It is suggested that the reaction system of noneequillibrum thermodynamically is a premise for the occurrence of magnetic field effects in an enzymic reaction.
出处
《南昌航空工业学院学报》
CAS
1996年第2期10-15,共6页
Journal of Nanchang Institute of Aeronautical Technology（Natural Science Edition）
关键词
葡萄糖
氧化酶
磁场效应
Glucose oxidase,Magnetic field effect(MEF),Flow injection analysis,Enzymic activity
