摘要
用海藻酸钠与磁粉作载体包埋-戊二醛交联制备磁性固定化酪氨酸酶(MIT),研究了弱磁场对MIT催化特性的影响。制得MIT活力达89U/g,SEM显示MIT形成有空孔网状结构,IR表明MIT包含了Fe3O4。MIT经弱磁场作用后其催化活性提高,增幅可达约35%左右,并且使MIT对酸碱和热稳定性增强,Michaelis常数有所下降。弱磁场处理有利于MIT与酚类底物反应速度的加快。
The magnetic immobilized tyrosinase （MIT） was prepared by using sodium alginate crosslinked with glutaraldehyde in the presence of magnetic powder. The effect of mild magnetic field on the catalytic characteristic for MIT were studied. The enzyme activity of the prepared MIT was 89 U· g^-1. The SEM indicated that MIT had a rougher and sharper surface and apparent pore structure network inside.Fe304 was found in IR spectrum. When the MIT was magnetized （M- MIT）, the catalytic activity was increased on increment about 35 % for MIT. The M - MIT was also of better pH stability and thermal - stability than that of MIT, and its Michaelis constants was decreased. After put into magnetic field, the reactive rate of catalyzing henolic compounds were quickened for MIT under the same conditions.
出处
《贵州工业大学学报（自然科学版）》
CAS
2006年第6期22-27,共6页
Journal of Guizhou University of Technology(Natural Science Edition)
基金
广东省自然科学基金(No.04020114)资助项目
关键词
磁效应
酪氨酸酶
固定化
催化活性
magnetic effect
tyrosinase
immobilization
catalytic activity
