摘要
目的:建立稳定的脑出血大鼠模型,并观察其行为学及组织结构变化。方法:采用Ⅶ型胶原酶0.5U/2.5"l生理盐水,在立体定位仪下注入大脑尾状核,然后在不同时间观察其行为学及形态学变化。结果:模型成功率80%,出血后6h大体形态可见明显出血灶,直径约3.0—3.5mm,且血肿大小、形态、部位相近;光镜下可见出血早期神经细胞水肿,白细胞浸润,后期神经胶质细胞、血管内皮细胞及神经细胞增生;大鼠行为学改变明显,并可持续到出血后7d。结论:此模型死亡率低,成功率高,血肿稳定,行为学改变明显,病理学改变符合临床。
Objective：To make a model of intracerebral hemorrhage （ICH） in rat and to study the changes in the behavior and tissue structure during absorption of the hematoma.Method：A model of ICH was established by stereotactically injection of 0.5U bacterial collagenaseⅦ per 2.5μl NS into caudate nucleus in rats. The behavior of rats with ICH was studied by measurement of Bederson score,beam-walking test,bilateral forepaws grasp and forelimb placing. The changes in histology were observed. Result： The successful ratio of establishment of this model was 80%.The behavioral changes were significant in experimental rats. There were obvious hematomas at 6h after hemorrhage and the size,shape and position of hematomas were stable, The diameters of hematoma were about 3.0-3.5mm. In the earlier period,the neuron cells were swelling. In the late period, a lot of glial cells, endothelial cells of vessel and neuron cells appeared around hematoma. Conclusion：The ICH model in rat by intracerebrally injection of collagenase was reproducible. The obvious behavioral changes and histological changes were similar to clinical patient with ICH.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2006年第11期977-979,I0001,共4页
Chinese Journal of Rehabilitation Medicine
关键词
胶原酶
脑出血
模型
行为改变
组织改变
bacterial collagenase Ⅶ
intracerebral hemorrhage
model
behavioral changes
histological changes
