摘要
目的研究解脲脲原体感染与男性不育的关系，探讨解脲脲原体（ｕｒｅａｐｌａｓｍａｕｒｅ－ａ－ｌｙｔｉｃｕｍ，Ｕｕ）引起男性不育的可能机制。方法用扫描电镜和免疫酶（ＰＡＰ法）染色观察解脲脲原体感染的不育患者精子。结果Ｕｕ阳性精子在扫描电镜下可见部分精子的头、尾部有明显颗粒状物质吸附，而Ｕｕ阴性的精子上未见任何颗粒吸附。Ｕｕ在扫描电镜下多呈卵圆形和球杆状，单个、成双或呈链状排列，与吸附于精子上的颗粒相似。３３份Ｕｕ培养阳性的精子涂片用抗Ｕｕ血清做ＰＡＰ免疫酶染色，证明其中２７份精子上（８１．８１％）有ＰＡＰ阳性的棕黄色Ｕｕ颗粒吸附，而２２份Ｕｕ阴性的精子涂片上未见任何ＰＡＰ阳性的棕黄色颗粒。结论大部分精液经Ｕｕ感染后，Ｕｕ可吸附于精子的表面，使精子的负荷增加，运动阻力增大。
ObjectiveTostudythecorelationbetweenureaplasmaurealyticuminfectionandmaleinfertility.MethodsThescanningelectronmicroscopyandPAPimmunoenzymatictechniquewereusedtodetectthespermsofinfertilemaleinfectedwithUu.ResultsBythescanningelectronmi-croscopy,wefoundmanysmalparticlesadherenttothehead,midpiece,andthetailofthespermsinfectedwithUu.TheshapeoftheparticleswasverysimilartothatofthepurifiedUu,buttherewerenotanysmalparticlesadherenttothespermsuninfectedwithUu.PAPstainingofthespermsof33infertilemalesinfectedwithUuwithanti-Uuantibodyasadiagnosticserumwasusedtoiden-tifytheseparticlesadheringtothesperms.PAPpositiveparticleswerefoundamong27cases(81.81%),mean-while,thespermsof22infertilemalesuninfectedwithUuwerealsostainedwiththesamemethod.Nopositiveparticlesadherenttothespermswerenoted.ConclusionsTheresultsshowedthattheadherenceofUumakesthespermsheavilyloadedandmotilitydecreased,andatlastleadstoinfertility.
基金
国家自然科学基金
关键词
解脲脲原体
不育症
免疫酶技术
扫描电镜
男性
UreaplasmaurealyticumInfertilityImmunoenzymatictechniqueScanninge-tectronmicroscopy
