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以Bar为选择标记的遗传转化体系的建立 被引量：1

Development of Transformation System with Selective Marker Bar Gene
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摘要 Bar基因是转基因商品化作物中应用最多的目标基因,也是水稻遗传转化中应用较多的选择标记基因,因此,建立以Bar基因为选择标记的通用和高效的遗传转化体系非常必要。本研究中,以籼型水稻明恢63为受体,Bar基因作选择标记,采用农杆菌介导的遗传转化法,将Bar基因转入其中,获得了一批转化子,初步建立了稳定、高效的水稻遗传转化体系。随后,以此体系为基础,将装有不同基因的两个载体pBar-1C*和pBar-1Ab进行了转化,分别从5400块和4800块愈伤组织中获得156个和115个抗性愈伤,其抗性愈伤率分别为2.8%和2.4%,分化率分别为80.7%和87.8%,由此说明,此转化体系相对稳定,且分化率高、抗性愈伤率也较高,可用于同类选择标记的遗传转化,加快水稻转基因育种步伐。 Bar gene is widely used in commercialized transgenic crops as a selective marker and also used in rice transformation. Therefore, the establishment of a kind of common and efficiency genetic transformation system is necessary. In the study, by using indica rice Minghui 63 as transformation receptor and Agrobacterium-mediated genetic method, Bar gene was transformed into Minghui 63. Thus, a stable and efficiency transformation system office was founded. Subsequently, on the basis of the system, the vectors pBar-1C^＊ and pBar-1Ab were transformed. 156 and 115 of resistant calli were obtained from 5 400 and 4 800 calli, respectively. Their transformation efficiency were 2.8% and 2.4%, and differentiation rates were 80.7% and 87.8%, respectively. These results suggest the transformation system is stable as well as efficiency, and good for transformation office with Bar gene as selective marker.
作者 唐微 朱名安
机构地区 湖北省郧阳医学院生化教研室
出处 《分子植物育种》 CAS CSCD 2006年第6期853-858,共6页 Molecular Plant Breeding
基金 科技部国家转基因植物研究与产业化专项（JY03-B-12）资助.
关键词 选择标记 BAR基因 农杆菌介导的遗传转化 水稻 Selective marker, Bar, A grobaeterium-mediated genetic transformation, Oryza sativa
分类号 S336 [农业科学—作物遗传育种]
  • 相关文献

参考文献14

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共引文献65

同被引文献20

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分子植物育种
2006年 第6期

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