摘要
为建立一个番茄遗传转化的稳定系统，本研究以“中蔬四号”品种为试验材料，采用含pBI121质粒的根癌农杆菌EHA105介导的方法研究了番茄遗传转化的相关因素。结果表明，外植体预培养2d，GUS染色效果最好，抗性芽诱导率最高为22．75％；在诱导和共培养基中都加入200～300μmol／L乙酰丁香酮时，GUS染色效果和抗性芽诱导率均优于其他组合；菌液OD600值为0．6时，外植体生长良好，GUS染色效果最佳，抗性芽诱导率最高为25．16％；最有利于抗性芽诱导的农杆菌侵染时间和共培养时间分别以5min和2d为宜。通过抗性植株的PCR检测，确定目的基因已整合到番茄基因组中，这为今后番茄的转化提供一些参考。
Using tomato variety ＂Zhong Shu No.4＂ and the Agrobaeterium strain EHA105 containing pBI121 plasmid as the materials, the paper studied the factors which influence Agrobaeterium-mediated transformation efficiency of the tomato so as to establish a stable transformation system. The results showed that GUS staining was best and the induction rate of the resistant buds was the highest （22.75%） when the explant were pre-eultured for 2 d. When 200-300 μmol/L acetosyringone were added to induction and co-culture media, both Gus staining and induction rate of resistant buds were better than other combinations. The explants grew well if they were infected by A grobacterium （OD600 ≈ 0.6） and GUS staining was the best and the induction rate of the resistant buds was the highest （25.16%）. Infection with A grobacterium and co-culture time that are suitable to induction of the resistant buds was 5 min and 2 d respectively. PCR test results to the resistant plants have indicated that the target gene have been integrated into the genome of tomato. This provides some references for the transformation of tomato in the future.
出处
《分子植物育种》
CAS
CSCD
北大核心
2013年第5期592-599,共8页
Molecular Plant Breeding
基金
山西省农业科技攻关项目(2009031102-2
20110311015-1)
山西省人事厅人才引进项目共同资助
关键词
番茄
农杆菌
遗传转化
Tomato, Agrobacterium, Genetic transformation
