摘要
以猪胰腺作为分离、提纯磷脂酶A_2的原料,研究了提取过程中的主要因素对酶活力的影响,其关键工艺条件为选择0.10mol/LNaCl等渗溶液与磷酸盐缓冲液(pH=6.0)作为萃取剂对猪胰进行匀浆,在50℃下恒温40min,pH=4.0酸处理后获得粗酶液。粗酶液经胰蛋白酶的激活与抑制,再经盐析、透析后,获得比活力为0.12U/mg、回收率34.6%、纯化倍数为31.7的磷脂酶A_2清液。
Pig pancreas were utilized to be the materials of extracting phospholipase A2 , the effect of main factors to enzyme activity of phospholipase A2 in the extracting process were researched in this article. The key technology condition were that 0.10mol/L NaCI and 0.03 mol/ L H3PO4 （pH =6.0） were chosen as extracting liquid to extract pig pancreas, crude enzyme liquid was obtained after dealing it with acid （PH=4.0） 40min at 50℃. Phospholipase A2 was gained after the crude enzyme liquid was activated and inhibit by trypsin, salted out, dialyzed, liquid specific activity was 0.12U/mg, recovery was 34.6% and the purification fold was 31.7 respectively.
出处
《黑龙江八一农垦大学学报》
2006年第5期51-54,共4页
journal of heilongjiang bayi agricultural university
基金
黑龙江省农垦总局科技攻关项目(HNKXV-05)。
关键词
磷脂酶A2
胰腺
提取
酶活力
phospholipase A2
pancreas
extraction
enzyme activity
