摘要
利用cDNA芯片技术分析陆地棉显性单基因突变的李氏超短纤维突变体(Li1li1)和其野生型(li1li1)纤维发育4 DPA(day post anthesis)的基因差异表达情况。RT-PCR验证4DPA芯片结果,得到15个差异表达的EST(expressed sequencetag),其中9个下调表达,即野生型(li1li1)中的表达量低于李氏超短纤维突变体(Li1li1)中的表达量;6个上调表达,即野生型(li1li1)中的表达量高于李氏超短纤维突变体(Li1li1)中的表达量。推测这些基因在棉花纤维伸长发育时期起了比较重要的作用。
Cotton fibers are single-celled seed triehomes of major economic importance. Numerous investigations at both the ultrastructural and biochemical level have shown that fiber ceils develop in four distinct but a little overlapping phases which are initiation, cell elongation （primary wall synthesis）, cell wall deposition and maturation. Factors regulated the rate and duration of cell expansion control the fiber morphological and important agronomic traits. The objective of the study was to illustrate the gene expression differences during the primary wall synthesis by using Ligon lintless mutant line （ Liilii ） and its wild line （liilii）, which the Ligon lintless mutant （Liilii ） is the dominant monogenic mutant and its dominant homogeneous genotype is not survivable. 1436 unique genes obtained by 5＇ single-pass sequencing more than 10 000 fiber cDNAs expressed sequence tags （ESTs） were made into genechips. The ovule and fiber compound of the Ligon lintless mutant line （Liilii ） and the wild line （liilii ） at 4 day post anthesis （DPA） were used for gene expression analysis in silica. The results validated by reverse transcription PCR （RT-PCR） , revealed that fifteen ESTs had differential expression. Among them, nine were down-regulation expression, the expression quantity being less in the wild line （ liilii ） than in the Ligon lintless mutant line （Liilii） . Six ESTs were up-regulatlon expression, the expression quantity being more in the wild line （ liilii ） than in the Ligon lintless mutant line （ Liilii）. The deduced function of the differential expression ESTs were associated with protein metabolism, energy/carbohydrate metabolism, environmental response/defense, cellular structure and organization, lipid metabolism transcription. These genes are speculated to play important roles in the cell elongation of cotton fiber.
出处
《作物学报》
CAS
CSCD
北大核心
2006年第11期1649-1655,共7页
Acta Agronomica Sinica
基金
国家自然科学基金(30471104
30270806)
国家重点基础研究发展规划(973规划)(2002CB111303)
教育部长江学者和创新团队发展计划
新世纪优秀人才项目(NCET-04-0500)
教育部科学技术重大研究项目(10418)
江苏省人才基金(BK2003414)
