期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

重组质粒pcDNA3．1IL-2/Fc对HBV preS2S DNA疫苗免疫作用的影响 被引量：2

Effect of pcDNA3.1IL-2/Fc as adjuvant on HBV preS2S DNA vaccination
原文传递
导出
摘要 目的探讨IL-2/Fc融合表达后对HBV preS2S基因疫苗诱导免疫反应的佐剂效应。方法采用HBV preS2S DNA疫苗作为基础免疫,重组质粒pcDNA3．1IL-2/Fc作为佐剂加强免疫BALB/c小鼠．采用0、2、4周的方案接种,检测各次接种后抗体水平。初次免疫后7周,测定免疫脾细胞的杀伤活性、增殖活性和细胞因子的分泌水平。结果pcDNA3．1IL-2/Fc作为佐剂在HBV preS2S注射3d后免疫组小鼠抗体滴度、免疫脾细胞的杀伤活性和增殖活性、T_H1型细胞因子的分泌水平,均比各对照组明显增强。结论IL-2/Fc是有效的HBV preS2S DNA疫苗佐剂之一。 Objective To study the adjuvant effect of fused gene IL-2/Fc on immune responses induced by HBV preS2S genetic vaccine. Methods BALB/c mice were immunized at 0, 2, 4 weeks as following： HBV preS2S genetic vaccine was chosen as the basic immunization, followed by the administration of pcDNA3. 1IL-2/Fc as adjuvant at the 3^rd day. Then anti-HBs titers, CIL cytotoxicity levels, proliferation of lymphoeytes and cytokines levels secreted by cultured splenoeytes were tested respectively. Results The humoral and cellular immune responses enhanced by pcDNA3. 1IL-2/Fc after HBV preS2S DNA vaccination in mice were dominant higher than control groups. Conclusion pcDNA3. IIL-2/Fc may be a potential genetic adjuvant.
作者 陈红梅 白雪帆 黄长形 李光玉 洪沙
机构地区 第四军医大学唐都医院感染科
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2006年第10期911-914,共4页 Chinese Journal of Microbiology and Immunology
关键词 HBV DNA 疫苗 IL-2 佐剂 HBV DNA vaccine IL-2 Adjuvant
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献9

  • 1陈红梅,白雪帆,黄长形,洪沙,王平忠,韦三华,王九平.IL-2/Fc融合基因真核表达载体的构建和表达[J].医学研究生学报,2004,17(1):12-14. 被引量：9
  • 2Cupps TR,Tibbles J,Hurni WM,et al.In vitro T cell immune responses to the preS2 antigen of the hepatitis B virus envelope protein in preS2 + S vaccine recipients.Absence of cross-reactivity of subtypes at a major T cell recognition site.J Immunol,1993,151(6):3353-3360.
  • 3Milich DR,McLachlan A,Chisari FV,et al.Immune response to the pre-S(1) region of the hepatitis B surface antigen (HBsAg):a preS(1)specific T cell responsse can bypass nonresponsiveness to the pre-S(2) and S regions of HBsAg.J Immunol,1986,137:315-322.
  • 4Bucher B,Francioli P,Geudelin B,et al.Immunogenicity of a recombinant preS2-containing hepatitis B virus vaccine.Eur J Clin Micribiol Infect Dis,1994,13:212-217.
  • 5Pillot J,Poynard T,Elias A,et al.Weak immunogenicity of the preS2 sequence and lack of circumventing effect on the unresponsiveness to the hepatitis B virus vaccine.Vaccine,1995,13(3):289-294.
  • 6Schmitt S,Glebe D,Alving K,et al.Analysis of the preS2 N-and Oglycans of the M surface protein from human hepatitis B virus.J Biol Chem,1999,274(17):11945-11957.
  • 7Sobotta D,Sominskaya I,Jansons J,et al.Mapping of immunodominant B-cell epitopes and the human serum albumin-binding site in natural hepatitis B virus surface antigen of defined genosubtype.J Gen Virol,2000,81(pt 2):369-378.
  • 8Machida A,Kishimoto S,Ohnuma H,et al.A polypeptide containing 55amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleotide acid bears the receptor of polymerized human as well as chimpanzee albumins.Gastroenterology,1984,86:910-918.
  • 9Park JH,Lee MK,Kim HS,et al.Targeted destruction of the polymerized human serum albumin binding site within the preS2 region of the HBV surface antigen while retaining full immunogenicity for this epitope.J Viral Hepat,2003,10(1):70-79

二级参考文献23

  • 1[1]McDonnell WM, Askari FK. DNA vaccines[J].N Engl J Med,1996,334(1):42-45.
  • 2[2]Lagging LM,Meyer K,Hoft D et al.Immrne responses to plasmid DNA encoding the hepatitis C virus core protein[J]. J Virol ,1995,69(9):5859-5863.
  • 3[3]Ulmer JB,Donnelly JJ,Paker SE et al.Heterologous protection against influenza by injection of DNA encoding a viral protein[J].Science,1993,259(5102):1745-1749.
  • 4[4]Wang B,Ugen ke,Srikantan V et al.Gene inoculation generates immune responses against human immunodeficiency virus type 1[J].Proc Natl Acad Sci USA,1993,90(9):4156-4160.
  • 5[5]Michel ML,Davis HL,Schleef M et al.DNA-mediated immunization to the hepatitis B surface antigen in mice:aspects of the humoral response mimic hepatitis B viral infection in humans[J].Proc Natl Acad Sci USA,1995,92(12):5307-5311.
  • 6[6]Jean P,Scheerlinck Y.Genetic adjuvants for DNA vaccines[J]. Vaccine,2001,19(17-19):2647-2656.
  • 7[7]Krudkall MS,Alper CA,Awdeh Z et al.The immune response to hepatitis B vaccine in humans: inheritance patterns in families[J].J Exp Med,1992,175(2):495-502.
  • 8[8]Cohen J.Naked DNA points the way to vaccines[J].Science,1993,259(5102):1691-1692.
  • 9[9]Musey L,Hughes J,Schacker T et al.Cytotoxic T-cell responses,viral load, and disease progression in early human immunodeficiency virus type 1 infection[J].N Eng1 J Med,1997,337(18):1267-1274.
  • 10[10]Geissler M,Gesien A, Wands JR. Inhibitory effects of chronic ethanol consumption on cellular immune responses to hepatitis C virus core protein are reversed by genetic immrnizations augmented with cytokine-expressing plasmids[J]. J Immunol,1997,159(10):5107-5113.

共引文献8

同被引文献12

引证文献2

二级引证文献4

中华微生物学和免疫学杂志
2006年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部