摘要
以乳酸—乙醇酸共聚物为载体,利用复乳—液中蒸发法制备生长激素释放肽-6(GHRP-6)微球。结果为包封率达100%,平均粒径3.45μm,回收率79%;在37℃p、H 7.0的生理等渗缓冲液中,首日释药19.19%,后以平均日释药3.23%的速度释药25 d。分别对分组家兔肌肉注射GHRP-6微球(2 mg/kg)、GHRP-6(2 mg/kg)、空微球和生理盐水,37 d后,GHRP-6微球组家兔累积增重分别比GHRP-6组、空微球组和生理盐水组高56.34%(P<0.01)、86.68%(P<0.01)和78.41%(P<0.01);采用RIA法测定家兔血清IGF-Ⅰ浓度,注射10 d后,GHRP-6微球组血清IGF-Ⅰ水平较注射前升高41.06%(P<0.05),注射20 d后,分别比GHRP-6组、空微球组和生理盐水组高27.53%(P>0.05)、123.88%(P<0.05)和275.66%(P<0.01)。试验结果表明:GHRP-6乳酸乙醇酸共聚物微球表现出明显的缓释特点,具有显著的促生长作用,有望发展成为新型长效制剂。
Sustained-releasing GHRP-6 microspheres were prepared by double emulsion-in hquid evaporation process, using biodegradable polylactic-co-glycolicacid as carrier and the drug release test of the microspheres was detected in vitro. The entrapment efficiency was 100%, mean particle size 3.45 μm and the yield above 79%. In Ph 7.0 physiological isotonic buffer solution, after 19.19% of GHRP-6 was released on the first day, the microspheres released at a rate of 3.23 % per day for more than 25 d （ r = 0.877 ）. Rabbits were divided into four groups with 8 rabbits in each group randomly. Rabbits were treated with GHRP-6 microspheres（2 mg/kg of BW）, GHRP-6（2 mg/kg of BW）, empty microspheres and saline by muscular injection. 37 days later, the mean accumulative weight gain of rabbits injected with GHRP-6 microspheres was 56.34%（P 〈 0.01）, 86.68%（P 〈 0.01）, 78.41%（P 〈 0.01）higher than that of rabbits injected with GHRP-6, empty microspheres and saline, respectively. Before and after injection, concentrations of sermn IGF- Ⅰ of rabbits were detected by mdioimmunoassay （RIA） using a human IGF-1 RIA kit. Concentrations of serum IGF- Ⅰincreased 41.06% （P 〈 0.05）in GHRP-6 microspheres group on the tenth day, 27.53% （ P 〉 0.05）, 123.88% （ P 〈 0.05）, 275.66% （ P 〈 0.01 ） higher than those groups injected with GHRP-6, empty mierospheres and saline respectively.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第5期560-563,573,共5页
Journal of Jilin Agricultural University
基金
国家自然科学基金资助项目(30270973)
关键词
乳酸-乙醇酸共聚物
微球
生长激素释放肽-6
家兔
poly （D, L-laetide-eo-glyeolide）
mierosphere
growth hormone releasing peptide-6
rabbit
