摘要
【目的】采用体外试验,研究了生长激素释放肽-2(GHRP-2)在不同处理浓度、不同处理时间和不同处理次数条件下对新生仔猪垂体细胞分泌生长激素(GH)的影响。【方法】分离1～3日龄新生仔猪腺垂体细胞,贴壁培养形成单层后,分别用0、10-12、10-11、10-10、10-9、10-8、10-7、10-6、10-5mol·L-1浓度的GHRP-2处理垂体细胞2h;用10-6mol·L-1浓度的GHRP-2处理垂体细胞5～180min;连续用10-6mol·L-1浓度的GHRP-2处理垂体细胞6次,每次1h。【结果】10-12～10-5mol·L-1浓度的GHRP-2都能作用于体外培养的垂体细胞,刺激垂体细胞分泌GH(P<0.01),10-12～10-8mol·L-1浓度之间差异不显著(P>0.05),10-7～10-5组极显著高于10-12～10-8mol·L-1(P<0.01),GHRP-2适宜浓度为10-6mol·L-1;GHRP-2能引起GH的快速分泌,在10～20min内就可使GH的分泌达到最大;在连续处理的前3次,GHRP-2能促进GH的分泌,从第4次开始,GH的分泌出现抑制状态,并随着处理次数的增加抑制状态更强。【结论】GHRP-2能够促进垂体细胞分泌GH,但其作用效果与处理剂量、时间和次数有关。
【Objective】This study was carried out to study the effect of growth hormone-releasing peptide-2 on growth hormone releasing of swine pituitary cells in vitro. 【Method】 Swine pituitary cells were isolated from three-day new born piglet and incubated with DMEM medium in the plating culture method. These cells were treated with GHRP-2 at different concentrations of 0, 10-12, 10-11, 10-10, 10-9, 10-8, 10-7, 10-6 and 10-5 mol·L-1 for two hours, respectively; Furthermore, the swine pituitary cells were treated with GHRP-2 of 10-6 mol·L-1 for 5 to 180 minutes. On the other hand, the swine pituitary cells were treated with GHRP-2 of 10-6 mol·L-1 for six times in succession, each time for one hour. 【Result】 GHRP-2 of 10-12 -10-5 mol·L-1 all could stimulate the pituitary cells incubated in vitro to excrete GH (P<0.01), and 10-12-10-8 mol·L-1 interclass was not significant (P>0.05). 10-7-10-5 mol·L-1 groups and 10-12-10-8 mol·L-1 groups was very significant (P>0.01). GH value was up to peak within 10 to 20 minutes. GHRP-2 could accelerate the release of GH in the first three treatments, however, the secretion was restrained after the fourth time and the negative influence increased by more treatments. 【Conclusion】 GHRP-2 may accelerate growth hormone release of the swine pituitary cells in vitro.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第12期2848-2852,共5页
Scientia Agricultura Sinica
基金
教育部长江学者和创新团队发展计划(IRTO555)项目
关键词
生长激素释放肽-2
猪腺垂体
原代细胞培养
生长激素
Growth hormone-releasing peptide-2
Swine pituitary
Primary cell culture
Growth hormone
