摘要
目的研究caveolin-1对胃癌细胞生物学行为的影响及作为基因治疗的候选基因可能性。方法用L ipofectin将caveolin-1基因稳定转染胃癌细胞系建立稳定表达caveolin-1的胃癌细胞系,并将空载体转染MGC803作为对照。采用western b lot检测转染及未转染的MGC803细胞中caveolin-1的表达情况。应用细胞计数、流式细胞术等检测caveolin-1对MGC803细胞生长的影响。结果转染后的MGC803细胞的caveolin-1的表达明显高于未转染细胞;细胞的群体倍增时间明显延长,细胞增殖周期明显延长,且阻滞于G0/G1期。结论Caveolin-1能抑制MGC803细胞的增殖,且将其阻滞于G0/G1期。
Objective To study the effect of caveolin-1 gene expression on the proliferation of human gastric adenocarcinoma cells, and to explore the possibility for its future usage in gene therapy. Methods The full-length caveolin-1 gene was stably transfeeted into the MGC803 cell line by lipofectin. The Pcl neo vector was transfected at the same time as mock control, The expression of caveolin-1 was detected by Western blot in both the caveolin-1 gene transfected MGC803 cells and the controls, The cell cycle was analyzed by flow cytometry. Results After transfected with caveolin-1, MGC803 cells significantly up-regulated the expression of caveolin 1 and extended their doubling time. The cell proliferation was inhibited and the cell cycle was arrested in the G0/G1 phase, Conclusion Caveolin-1 can inhibit the proliferation of MGC803 cells and induce cell cycle arrest in G0/G1 phase.
出处
《中国医师杂志》
CAS
2006年第10期1323-1325,共3页
Journal of Chinese Physician
基金
国家自然科学基金(30270684)
湖南省杰出中青年专家专项基金(02JJYB004)资助项目
