摘要
目的:探索降钙素基因相关肽(CGRP)相关的干预措施对胃痛觉过敏的影响,了解CGRP在胃痛觉过敏形成过程中发挥的作用．方法:成年SD古大鼠,均植入胃内气囊．观察伤害性扩张或CGRP iv对大鼠疼痛阈值的影响:观察由上述措施诱发内脏过敏的大鼠在给予CGRP受体特异性拮抗剂hCGRP8-37后疼痛阈值的变化:观察不同剂量CGRP和hCGRP8-37对疼痛阈值的影响．结果:CGRP iv后胃疼痛阈值为11．7±2．6 mmHg,对照组疼痛阈值为19．2±2．0 mmHg,生理盐水对照组则为18．3±2．5 mmHg,实验组与其他两组比较尸均<0．05．CGRP使大鼠的疼痛阈值降低．hCGRP8-37能逆转伤害性扩张和CGRP引起的内脏敏感性增高,该作用呈剂量依赖性(r=0．821,P<0．01)．结论:胃扩张刺激能引起胃敏感性增高,在此过程中CGRP具有重要的作用．
AIM： To explore the role of calcitonin generelated peptide （CGRP） in gastric distentioninduced visceral pain in conscious rats. METHODS： Balloons were implanted into the stomach of 24 male Sprague Dawley rats. In this study we observed the following outcomes： （1） effects of noxious distention or CGRP intravenous injection on gastric sensitivity; （2） effects of a specific CGRP receptor antagonist, hCGRP8-37 on gastric hypersensitivity; （3） relationship between the doses of CGRP ＆ hCGRP8-37 and their effects on gastric sensitivity. Gastric sensitivity was evaluated with gastric pain threshold determined by electromyography （EMG） of the neck muscles. RESULTS： After intravenous injection of CGRP, the values of gastric pain threshold were 11.7 ± 2.6, 19.2 ± 2.0, and 18.3 ± 2.5 mmHg in experiment group, operation control group, and normal saline group, respectively, and there was significant difference between the former one and latter two （P 〈 0.05）. CGRP down-regulated the gastric pain threshold, hCGRP8-37 reversed the harmful gastric distention, and inhibited the gastric hypersensitivity in a dose-dependent manner （r = 0.821, P 〈 0.01）. CONCLUSION： Gastric distention can stimulate the gastric hypersensitivity, in which CGRP plays an important role.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第25期2484-2487,共4页
World Chinese Journal of Digestology
