摘要
用荧光光谱、紫外可见吸收光谱和圆二色光谱技术研究了生理条件下硫堇(TH)与牛血清白蛋白(BSA)的结合反应。结果表明,硫堇借静电力与牛血清白蛋白发生相互作用并对牛血清白蛋白的荧光以静态方式猝灭,其结合常数为1.155×105L.mol-1、结合位点数为1.835、结合距离2.865 nm。同步荧光和圆二色技术证实TH对BSA的构像有影响。
Fluorescence spectroscopy in combination with circular dichroism and UV-Vis absorbance spectrum were employed to investigate the binding of thionine and bovine serum albumin system under physiological conditions. The experimental results showed that electrostatic interaction played a major role in the interaction, thionine could quench the fluorescence of BSA with a static quenching mechanism. The apparent binding constant/number of binding sites were 1. 155× 105 L·mol^-1/1. 835 for TH-BSA, respectively. The distance between BSA and TH was estimated to be 2. 865 nm. Synchronous fluorescence spectra and circular dichroism of BSA in the presence of thionine indicated that the conformations of bovine serum albumin had been changed.
出处
《化学与生物工程》
CAS
2006年第10期60-62,共3页
Chemistry & Bioengineering
基金
教育部重点科研项目(206096)
湖北省教育厅重大科研项目(Z200522002)
中药生化技术湖北重点实验室开放基金项目(20040201)
关键词
相互作用
硫堇
牛血清白蛋白
荧光光谱
圆二色光谱
interaction, thionine
bovine serum albumin fluorescence spectrum circular dichroism
