摘要
为探讨腰椎间盘实出患者之髓核分子量级超微结构的形态，作者经对手术证实３例腰椎间盘突出症患者的髓核和１例鲜尸体的髓核应用原子力显微镜，进行了超微结构研究。结果表明两者髓核中的胶原束，胶原纤维和原胶原纤维呈不同排列，蛋白多糖的颗粒分子形态与正常亦不一，胶原纤维形成原胶原纤维网孔附着蛋白分子及蛋白多糖，ＤＮＡ吸附在纤维细胞核ＲＮＡ单链上，呈双链、多链三维螺旋结构形态。原胶原纤维呈带状结构（ＢａｎｄｅｄＳｔｒｕｃｔｕｒｅ）６４～７８ｎｍ，退变髓核胶原纤维化合并钙化，其含量明显高于正常（Ｐ＜０．０５）。提示腰椎间盘受到长时间的伤害，使胶原纤维损害断裂导致出现扭结现象，失去正常抗阻力。
Inordertoinvestigatethemorphologyofultramicrostructureinmolecularlevelofnucle-usherniations,theauthorsstudiedtheultramicrostructureofthreecasesofnucleusherniationsoflum-barintervertebraldiscwhichwereprovedbyoperationsandonecaseofnormalnucleuspulposusoffreshcorpsebyusingofatomicforcemicroscope.Theresultsshouedthatthearrangementofcolagenbundle,colagenfiberandprocolagenfiberwerediferentbetwenthetwoconditions,andthemorphol-ogyofmolecularparticlesofproteoglycaninnucleusherniationswasdiferentfromthatinthenormalcondition.Intheconditionofnucleusherniations,thecolagenfiberformedprocolagenfiberreticularporousadheringtothemoleculesofproteinandproteoglycan,andtheDNAadheringtothemonochainofRNAinfiberalcelnuclei,intheformofbichainandmultichainthreedimensionshelixstructures.Thebandedstructure(64～78nm)oftheprocolagenfiber,andthecolagenationandcalcificationofthedegeneratednuclenspulposuses,thequantityofwhichwassignificantgreaterthannomal(P<0.05)in-dicatedthatthechronicinjuryoflumbarintervertebralnucleuspulposusesgaverisetotheinjuryandbreakofthecolagenfiberwhichresultedintwist(tangle)phenomenonsothatnucleuspulposuseslosttheirnormalresistancestrength.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1996年第12期754-757,共4页
Chinese Journal of Surgery
