摘要
选用健康成年雄性SD大鼠,用不同剂量(10μg·kg-1·d-1,25μg·kg-1·d-1,50μg·kg-1·d-1)的雷公藤甲素预处理5d后,立体定位海马注射脂多糖(LPS),采用免疫组织化学染色方法观察海马CA3区环氧化酶2(COX-2)和诱导型一氧化氮合酶(iNOS)的表达变化。结果发现:与注射生理盐水对照组比较,注射LPS可引起海马CA3区的COX-2和iNOS显著表达,而雷公藤甲素(50μg·kg-1·d-1)可明显下调LPS诱导的COX-2和iNOS的表达,其抑制程度与药物剂量呈正相关。本实验结果提示雷公藤甲素对中枢神经系统中LPS诱导的COX-2和iNOS的表达有明显的抑制作用。
Immunohistoehemistry staining was used to analyse the expression of eyelooxygenase-2 （ COX-2 ） and inducible nitric oxide synthase （iNOS） in hippoeampal CA3 regions. In the present study, rats were injected intraperitoneally with triptolide at different doses （ 10 μg·kg^-1·d^-1 , 25 μg·kg^-1·d^-1, 50μg·kg^-1·d^-1 ）, prior to the injection of 4 μg/4μl lipopolysaeeharide （LPS） through hippocampus. The results showed that compared to saline-controlled group, injection of LPS intrahippoeampus could induce significant expression of iNOS and COX-2 in hippoeampus CA3 regions. However, Triptolide （50 μg·kg^-1·d^-1 ） markedly down-regulated LPS-indueed COX-2 and iNOS protein expression in hippoeampus , and this effect was positively correlated with triptolide doses. Our findings indicated that triptolide can obviously down-regulate LPS-indueed COX-2 and iNOS expression in central nervous system.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第5期521-524,共4页
Chinese Journal of Neuroanatomy
基金
高等学校博士学科点专项科研基金资助课题
关键词
雷公藤甲素
脂多糖
环氧化酶2
诱导型一氧化氮合酶
炎症
triptolide, lipopolysaccharide, cyelooxygenase-2, inducible nitric oxide synthase, inflammation
