摘要
目的研究不同采收时间和不同炮制方法的木瓜药材水溶性部位高效液相色谱(HPLC)指纹图谱,为木瓜的规范化种植与合理应用提供依据。方法以绿原酸为参照物,采用HPLC法测定分析各指纹图谱,色谱条件:C18(Krom asil C1810 nm^5μm,4.6 mm×250 mm)色谱柱,流动相:(A)乙腈-甲醇(6:4)(B)0.05%磷酸梯度洗脱,0~12 m in(10%~30%A,90%~70%B),12~30 m in(30%~50%A,70%~50%B),30~35 m in(50%~70%A,50%~30%B),35~45m in(70%~90%A,30%~10%B),45~50 m in(90%~60%A,10%~40%B),50~60 m in(60%~10%A,40%~90%B),60~70 m in(10%A,90%B);流速0.8 m l.m in-1,检测波长283 nm。结果木瓜水溶性部位的指纹图谱有较好的相关性。分析表明,木瓜不同采收时间的样品分为3类—第2,3,6,9批归为一类,有13,17,19 m in3个,共有峰;第1,5,8批归为一类,出峰各有5个,有13,17,19 3个共有峰;第4,7归为一类,有17,19两个共有峰。其中又以第5批采收时间最好;不同炮制方法以晒的方法所含峰数目和含量要高;不同存放时间以存放时间短的较好。结论该研究有助于木瓜的质量控制、采集和应用。
Objective To study the HPLC fingerprint of the water-soluble part of Fruit of Chaenomeles of different collected time and processed method, and to provide application and GAP basis for the herb.. Methods HPLC with UV detector was used to analyze the patterns of fruit of Chaenomeles. Chlorogenic acid was used as standard substances, C18 （ Kromasil C18, 10 nm - 5 μm,4.6 mm×250mm） column was used with a mobile phase of （A） acetonitrile-methonal （6：4） （B） 0. 05 %phosphoric acid gradient elution , 0-12 min （10% -30%A, 90% -70%B） ,12-30 min （30% -50 %A ,70% -50%B） ,30 -35 rain （50%-70%A ,50%-30%B） ,35-45min （70% -90%A ,30% - 10%B） ,45 -50 min （90%-60%A ,10%-40%B） , 50 -60 min（60% - 10% A , 40% -90% B） , 50 -60 min（ 10 %A ,90 % B） ;The flow rate was 0.8 ml· min^-1 ,The wavelength of deteetor was set at 283 nm. Results The fingerprint of fruit of Chaenomeles was set up and the fingerprint of them showed an excellent eorrelationship. Conclusion The study is helpful for the quality eontrol, the gathering and applieafion of Chinese flowering quince.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第10期1869-1871,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家"十五"科技攻关项目(No.2001BA701A60-12)
