摘要
应用增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）单克隆抗体ＰＣ１０，以Ｓ－Ｐ免疫组织化学染色方法观察分析ＰＣＮＡ在涎腺混合瘤、混合瘤细胞生长活跃型、恶性混合瘤、粘液表皮样癌中的表达分布情况。评价不同组织类型的涎腺肿瘤的增殖潜能及同一肿瘤组织中不同细胞成分的增殖活性。结果表明：恶性混合瘤有较高的ＰＣＮＡ增殖指数，与混合瘤细胞生长活跃型及混合瘤相比均有极显著性差异（Ｐ＜０．０１）；低分化粘液表皮样癌与高分化型相比有极显著性差异（Ｐ＜０．０１）。结论：ＰＣＮＡ是肿瘤细胞增殖的有效标记物；可作为恶性混合瘤的诊断指标；ＰＣＮＡ增殖指数可作为粘液表皮样癌组织学分级的重要参数。
The expression of proliferating cell nuclear antigen (PCNA) in 70 cases of common benign and malignant salivary gland tumours was studied using immunohistochemical method. The percentage of positive cells in totally counted tumour cells was defined as proliferating index (PI). Malignant mixed tumour had a higher PI (31.63±7.80) with significance difference ( P <0.01) compared with active proliferation mixed tumour (PI=7.15±4.25) and mixed tumour (PI=4.50±3.03). It indicated that the expression of PCNA may be an adjuvant diagnostic criterion for malignant mixed tumor. A significant difference of PI was also observed between low and high-grade mucoepidermoid carcinoma (PI=11.2±6.60 and 32.0±8.51, P <0 01), so PI may be an important parameter for the histological grade of mucoepidermoid carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
1996年第6期611-614,共4页
Journal of China Medical University
关键词
涎腺肿瘤
增殖细胞核
抗原
免疫组化
proliferating cell nuclear antigen
salivary gland tumour
immunohistochemistry stain
