摘要
健康成年杂种狗20只,随机分为3组:假手术对照组(n=6)、急性胰腺炎组(n=8)和胰腺炎用氯喹肌注顶处理组(n=6).胰腺炎组和氯喹组由主胰管注射自身胆汁1 ml/kg复制急性出血坏死性胰腺炎模型。结果表明,胰腺炎组肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)中肺泡Ⅱ型细胞和多形核白细胞显著增加,BALF及其细胞磷脂总量下降,其中溶血卵磷脂比例增高,卵磷脂和双饱和卵磷脂比例降低.肺匀浆磷脂总量无显著变化,但溶血卵磷脂增加,卵磷脂和双饱和卵磷脂减少.BALF及从中萃取的磷脂的表面活性显著降低.预先肌注氯喹,能显著改善BALF细胞分类和部分磷脂成分的变化,但对BALF及其磷脂的表面活性无显著影响.提示胰腺炎狗肺表面活性物质的损伤与磷脂酶A_2有关,氯喹有防治作用。
Twenty mongrel dogs were used and randomly divided into three groups: sham-operated controls, dogs with induced acute hemorrhagic pancreatitis (AHP) and those treated with chloroquine (Ch1) before AHP was induced (AHP+Ch1). AHP was induced by injcction of auto-bile 1 ml/kg into the main pancreatic duct. The results revealed that in AHP, bronchoalveolar lavage fluid (BALF) cell count increased significantly, particularly the alveolar typeⅡ cells and polymorphonuclear leukocytes (PMNs). BALF and cell total phospholipid content (TPL) decreased significantly, of which lysophos-phatidylcholine (LPC) increased, phosphatidylcholine (PC) and dipalmitoly phosphatidylcholine (DPPC) decreased. Lung homogenate showed no change in TPL, but had increased percentage of LPC and decreased percentage of PC and DPPC. Surface activity of BALF and its extracted phospholipid decreased significantly in AHP. Pretreatment with chloroquine can obviously prevent some of the changes in BALF cell types and in percent composition of phospholipid, but had no impact on PS activity. It is suggested that PLA2 participates in developments of pulmonary surfactant system defects and chloroquine has potential value in the treatment of acute lung injury that follows acute pancreatits.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第1期10-14,共5页
Academic Journal of Second Military Medical University
关键词
胰腺炎
表面活性物质
肺
氯喹
acute pancreatitis
pulmonary surfactant
chloroquine
