摘要
为验证气提式三重循环生物膜反应器处理甾体雌激素的效能，建立了一套SPE／HPLC／MS／MS分析方法，对反应器进、出水中的甾体雌激素——甾酮（E1），17β-雌二醇（E2），雌三醇（E3）以及17α-乙炔基雌二醇（EE2）进行检测．该方法的加标回收率为88％～103％，精密度为4％～9％．该方法对E1，E2，E3和EE2的进水定量限（LOQ）分别为0．7，0．8，0．9和0．5ng／L；对出水的定量限分别为2．0，1．0，2．0和1．0ng／L．CODCr和氨氮容积负荷最高为7．5和1．6kg／（m^3·d），出水依然能够保持稳定．进水pH稳定在9．0～10．5，而反应器的pH一直稳定在9．0以下，体系对pH变化造成的冲击比较适应．同时，反应器内形成了稳定的NO2^- -N积累，CODCr和氨氮的去除率分别达到了70％和73％．
An analytical method performed by solid-phase extraction （SPE） system with high pressure liquid chromatography and tandem mass spectrometry （HPLC/MS/MS） was developed for validating the efficiency of the triplet loop airlift biofilm reactor （TLABR）. The steroid estrogens, including estrone （E1）, 17β-estradiol （E2）, estriol （E3） and 17α-ethynylestradiol （EE2）, of influent and effluent of the bioreactor were determined by this method. The average recoveries ranged from 88% to 103% and the precision of the method ranged from 4% to 9%. The limit of quantification （LOQ） was determined at 0.7 ng/L for E1, 0.8 ng/L for E2, 0.9 ng/L for E3, and 0.5 ng/L for EE2 in influent, and 1.0 ng/L for E2 and EE2, and 2.0 ng/L for E1 and E3 in effluent. The effluent could still remain stable, when the influent volume load of CODer and NH3-N reached as high as 7.5 and 1.6 kg/（m^3·d） respectively. The influent pH was generally kept at 9.0 - 10.5, and the pH within the reactor remained stable at below 9.0. Therefore, the system could adapt to the impact of pH. Also, stable accumulation of NO2^- -N was formed in the reactor, and the removal rates of CODer and ammonia reached 70% and 73% , respectively.
出处
《环境科学研究》
EI
CAS
CSCD
北大核心
2006年第4期56-60,共5页
Research of Environmental Sciences
基金
北京市自然科学基金资助项目(8042007)
