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血管内皮生长因子C及其受体3mRNA在上皮性卵巢癌组织的表达及意义

The mRNA expression of vascular endothelial growth factor C and its receptor 3 in human epithelial ovarian carcinoma.
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摘要 目的探讨血管内皮生长因子C(VEGF-C)及其受体3(VEGFR-3)mRNA在上皮性卵巢癌组织的表达及与癌周淋巴管生成及淋巴转移的关系。方法2003-04-2004-06第三军医大学附属西南医院采用RT-PCR方法,检测VEGF-C及VEGFR-3mRNA在良、恶性上皮性卵巢肿瘤组织的表达及组织化学淋巴管染色并计数,分析其与VEGF-CmRNA表达及淋巴转移的关系。结果VEGF-CmRNA和VEGFR-3mRNA在卵巢良、恶性肿瘤的表达差异有显著性。VEGF-CmRNA表达阳性及有淋巴结转移组的癌组织淋巴管密度分别大于VEGF-CmRNA表达阴性及无淋巴结转移组的癌组织,二者差异均有显著性。结论VEGF-CmRNA在上皮性卵巢癌组织的表达上调导致了癌周淋巴管生成,促进了肿瘤的淋巴道转移。 Objective To study the mRNA expression of vascular endothelial growth factor C（VEGF-C） and its receptor 3 （VEGFR-3） ,in addition the relationship between them and the peri-tumor lymphangiogenesis, lymphatic metastasis in human epithelial ovarian carcinoma was also studied. Methods The expression of VEGF-C mRNA and VEGFR-3mRNA in benign and malignant epithelial ovarian tumors was detected by RT-PCR. Analyse the relationship between the lymphatic vessel density and the expression of VEGF-CmRNA and lymphatic metastasis by enzyme-histochemistry staining and lymphatics counting. Results The expression of VEGF-CmRNA and VEGFR-3mRNA was significantly higher in ovarian carcinomas than those in benign ovarian tumors, Lymphatic density in VEGF-CmRNA positive group and lymphatic metastasis group was significantly higher than those in VEGF-CmRNA negative group and non-lymphatic metastasis group respectively. Conclusion Up-regulation of VEGF-CmRNA in epithelial ovarian carcinoma facilitates the peri-tumor lymphangiogenesis and lymphatic metastasis.
作者 王玉珍 梁志清 王丹 常青 史常旭
机构地区 第三军医大学附属西南医院妇产科 现在解放军第
出处 《中国实用妇科与产科杂志》 CAS CSCD 北大核心 2006年第10期746-749,共4页 Chinese Journal of Practical Gynecology and Obstetrics
基金 国家自然科学基金资助项目(编号30371484)
关键词 卵巢癌 血管内皮生长因子C 淋巴管生成 淋巴道转移 Ovarian carcinoma Vascular endothelial growth factor C Lymphangiogenesis Lymphatic metastasis
分类号 R737.31 [医药卫生—肿瘤]
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参考文献9

  • 1Weidner N.Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors[J].Breast Cancer Res Treat,1995 (2),36:169-180.
  • 2Karkkainen MJ,Marika J,Alitalo K.Lymphatic endothelium:a new frontier of metastasis research[J].Nat Cell Biol,2002,4 (1):E2-5.
  • 3Hashimoto I,Kodama J,Seki N,et al.Vascular endothelial growth factor-C expression and its relationship to pelvic lymph node status in invasive cervical cancer[J].Br J Cancer,2001,85(1):93-97
  • 4Skobe M,Hawighorst T,Jackson DG,et al.Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis[J].Nature Med,2001,7(2):192-198.
  • 5Mandriota SJ,Jussila L,Jeltsch M,et al.Vascular endothelial growth factor-C-mediated lymphangiogenesis promotes tumour metastasis[J].EMBO,2001,20(4):672-682.
  • 6王玉珍,梁志清,龙玲,王丹.血管内皮生长因子C、D在上皮性卵巢癌的表达及与腹股沟和腹膜后淋巴结转移的关系[J].第三军医大学学报,2004,26(11):977-980. 被引量：9
  • 7Makinen T,Veikkola T,Mustjoki S,et al.Isolated lymphatic endothelial cells transduce growth,survival and migratory signals via the VEGF-C/D receptor VEGFR-3[J].EMBO,2001,20 (17):4762-4773.
  • 8Joukov V,Kumar V,Sorsa T,et al.A recombinant mutant vascular endothelial growth factor-C that has lost vascular endothelial growth factor receptor-2 binding,activation,and vascular permeability activities[J].J Biol Chem,1998,273(12):6599-6602.
  • 9Ono Y,Nakajima T,Saku T.Vascular invasion of O-1N,hamster squamous cell carcinoma with high potential of lymph node metastasis:ultrastructural comparison between lymphatics and blood vessels[J].Pathol Int,1998,48(4):254-264.

二级参考文献9

  • 1[1]Joukov V, Pajusola K, Kaipainen A, et al. A novel vascular endothelial growth factor, VEGF-C, is a ligand for the Flt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases[J]. EMBO J, 1996, 15(2): 290-298.
  • 2[2]Achen M G, Jeltsch M, Kukk E, et al. Vascular endothelial growth factorD (VEGF-D) is a ligand for the tyrosine kinases VEGF receptor 2 (Flk1)and VEGF receptor 3 (Flt4) [J]. Proc Natl Acad Sci USA, 1998, 95(2):548 - 553.
  • 3[3]Mandriota S J, Jussila L, Jeltsch M, et al. Vascular endothelial growth factor-C-mediated lymphangiogenesis promotes tumour metastasis[ J ]. EMBO J, 2001, 20(4): 672 - 682.
  • 4[4]Skobe M, Hawighorst T, Jackson D G, et al. Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis[J]. Nat Med,2001, 7(2): 192- 198.
  • 5[5]Stacker S A, Caesar C, Baldwin M E, et al. VEGF-D promotes the metastatic spread of tumor cells via the lymphatics[J]. Nat Med, 2001, 7(2):186- 191.
  • 6[6]Bunone G, Vigneri P, Mariani L, et al. Expression of angiogenesis stimulators and inhibitors in human thyroid tumors and correlation with clinical pathological features[J]. Am J Pathol, 1999, 155(6): 1967 - 1976.
  • 7[7]Tsurusaki T, Kanda S, Sakai H, et al. Vascular endothelial growth factorC expression in human prostatic carcinoma and its relationship to lymph node metastasis[J]. Br J Cancer, 1999, 80( 1 - 2): 309 - 313.
  • 8[8]Nakamura Y, Yasuoka H, Tsujimoto M, et al. Prognostic significance of vascular endothelial growth factor D in breast carcinoma with long-term follow-up[J]. Clin Cancer Res, 2003, 9(2): 716-721.
  • 9[9]Salven P, Lymboussaki A, Heikkila, et al. Vascular endothelial growth factors VEGF-B and VEGF-C are expressed in human tumors [ J ]. Am J Pathol, 1998, 153(1): 103-108.

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中国实用妇科与产科杂志
2006年 第10期

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