摘要
目的探讨VEGF-D在乳腺癌中的表达的临床意义及与转移和预后的关系。方法运用免疫组化S-P法和原位杂交方法。结果免疫组化:115例乳腺癌VEGF-D阳性表达率79.1%(91/115),VEGF-D表达与淋巴结转移有关,P=0.003,与年龄、大小、临床分级无关,(P=0.647,P=0.694,P=0.133);VEGF-D阳性组LVD值为(17.94±5.24)明显高于VEGF-D阴性组(13.25±4.65),(P=0.018),VEGF-D阳性组患者的5年生存率明显低于阴性组,P=0.0024。原位杂交:乳腺癌中VEGF-DmRNA阳性表达率为69.4%与蛋白表达一致率为78.6%。结论VEGF-D与乳腺癌的淋巴管形成、转移和预后密切相关。
[Objective] To investigate the protein and mRNA expression of VEGF-D in breast cancer and to find their correlation with prognosis and metastasis. [Methods] High sentitive S-P immunohistochemical method and in situ hybridization were used to detect the protein and mRNA expression of VEGF-D in 115 breast cancer specimens. [Results] Immunohistochemistry revealed that among the 115 cases, the positive rates of VEGF-D was 79.1%（91/115）. There was a significant correlation between the expression of VEGF-D protein and lymphatic metas- tasis （P =0.003）. However, it was not correlated with the age, the size of tumor and clinical stages （P 〉0.05）. The mumber of VEGFR-3 positive vessels in lymph node metasis group （17.94±5.24） was significantly higher than that in nonmetastatic （13.25±4.65） （P=0.018）. The postoperative survival period was significantly shorter in VEGF-D positive expression group comparing with that in negative one （P =0.0024）. The result of the in situ hybridization, the positive rate of VEGF-DmRNA was 69.4%, which followed closely that of protein-expression. [Conclusion] VEGF- D may play important roles in tumor lymphayenesis, metastasis, and prognosis of breast cancer.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第18期2785-2788,共4页
China Journal of Modern Medicine
