摘要
目的探讨葛根素对大鼠视网膜缺血再灌注损伤中核转录因子-κB(neuclearfactor-kappaBNF-κB)表达的抑制及对视网膜的保护作用。方法建立大鼠视网膜缺血再灌注模型,分缺血再灌注组、缺血再灌注+葛根素组和对照组。每组按再灌注时间的不同分为缺血再灌注1、6、12、24、48及72h组。以免疫组织化学法检测各期视网膜中NF-κB的表达,同时记录各期大鼠ERGa、b波波幅,通过透射电镜观察各期视网膜的超微结构。结果在缺血再灌注组,NF-κB在再灌注6h后开始表达,24h表达最强,以后逐渐下降。在缺血再灌注+葛根素组,在再灌注6h未见NF-κB的表达,再灌注12h后开始有表达,24h表达最强,但明显低于缺血再灌注未处理组同期NF-κB的表达。对照组中未见明显的NF-κB的表达。同时,缺血再灌注+葛根素组各时期ERGa、b波波幅高于缺血再灌注组而低于对照组。视网膜超微结构显示缺血再灌注+葛根素组细胞受损情况明显好于缺血再灌注组。结论葛根素对大鼠视网膜缺血再灌注损伤中NF-κB的表达具有抑制作用。
[Objective] To study the effect of Puerarin on the expression of NF-κB in rat＇s ischemia-reperfusion injuried retina and its protection on rat＇s retina. [Method] The model of retinal ischemia-reperfusion was constructed by ligationed the left side of artery, the rats were devided into ischemia-reperfusion group, ischemia-reperfusion and Puerarin group and control group, each including 1 h, 6 h, 12 h, 24 h, 48 h and 72 h. Immunohistochemistry was used to detect the expression of NF-κB protein in different stages of rat＇s retina. The ERG were examined in different stages, at the same time, the ultrastructure of retina were examined in different stages. [Result] In ischemia-reperfusion group, NF-κB began to express after reperfusion for 6 h and increased gradually, it reached the highest level after reperfusion for 24 h. In ischemia-reperfusion and puerarin group, NF-κB could be detected at 12 h, which expression was strongest at 24 h. But it was lower than the same stage of ischemia-reperfusion group. There was no NF-κB expressed in control group. The relative ERG a, b wave of ischemia-reperfusion and Puerarin group was higher than ischemia-reperfusion group, but lower than the control group. The uhrasturcture indicateded the damage of retina cells of ischemia-reperfusion and Puerarin group was lighter than ischemia-reperfusion group. [Conclusion] The Puerarin can inhibit the expression of NF-κB in rat＇s ischemia-reperfusion injuried retina and protect it.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第18期2725-2728,共4页
China Journal of Modern Medicine
