摘要
目的探讨肠内免疫营养(E IN)对烧伤后肠黏膜损伤的保护作用。方法采用30%总体表面积Ⅲ度烫伤大鼠模型。64只W istar大鼠采用随机数字表法分为E IN组和标准肠内营养(EN)组,另取8只大鼠作为伤前对照。E IN组和EN组给予等热量肠内营养液。分别取伤前对照及EN组、E IN组伤后1、4、7和10 d检测肠黏液分泌型免疫球蛋白A(sIgA)浓度及血浆二胺氧化酶(DAO)活性和肿瘤坏死因子α(TNFα)含量的变化,逆转录聚合酶链反应(RT PCR)方法检测肠黏膜组织TNFαmRNA表达。结果烫伤后EN组和E IN组肠黏液sIgA浓度、血浆DAO活性、TNFα含量和肠黏膜组织TNFαmRNA表达均比伤前显著升高(P<0.05或P<0.01);E IN组肠黏液sIgA浓度、血浆DAO活性和TNFα含量在伤后4、7和10 d,肠黏膜组织TNFαmRNA的表达于伤后1、4、7和10 d均比EN组显著降低(P<0.05或P<0.01)。结论与标准EN相比,E IN可明显减轻严重烫伤后肠黏膜损伤,其机制与增加肠黏液sIgA浓度、降低肠黏膜组织TNFαmRNA的表达有关。
Objective To investigate the protective effects of enteral immunonutrition on intestinal mucosa injury following severe burn. Methods Sixty-four Wistar rats with 30% total body surface area （TBSA） full-thickness burn were randomly divided into enteral nutrition （EN, n=32） group and enteral immunonutrition （EIN, n=32） group. Another 8 Wistar rats were used as normal control group. The concentration of secretory immunoglobulin A （sIgA） in intestinal mucosa, the blood levels of diamine oxidase （DAO） activity and tumor necrosis factor-α （TNF-α） were determined, and the expression of TNF-α mRNA in intestinal mucosa was determined by reverse transcription polymerase chain reaction （PT - PCR） in control group and on 1 st, 4 th, 7 th, and 10 th day postburn in EN and EIN group. Resulls Compared with that of before injury, the blood levels of DAO activity, TNF -α content, the concentration of sIgA and the expression of TNF -α mRNA in intestinal mucosa were significantly increased in both EN and EIN groups （P〈0.05 or P〈0.01）. However, the blood levels DAO activity, TNF-α content, the concentration of sIgA on 4 th, 7 th and 10 th postinjury days and the expression of TNF-α mRNA in intestinal mucosa on 1 st, 4 th, 7 th and 10 th days postinjury in EIN group were significantly lower than those in EN group （P〈0. 05 or P〈0.01）. Conclusion EIN can ameliorate the damage of the intestinal mucosa in burned rats compared with EN group. This might be related to an increase in the sIgA level and reduction of the expression of TNF -α mRNA in intestinal mucosa.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第10期609-612,共4页
Chinese Critical Care Medicine
基金
安徽省教育厅自然基金资助项目(2003kj201)
关键词
肠内营养
免疫营养
肠黏膜
烧伤
enteral nutrition
immunonutrition
intestinal mucosa
burn
