摘要
测定烫伤大鼠早期口服谷氨酰胺（Ｇｌｎ）后血和组织中的游离氨基酸（ＦＡＡ）、二胺氧化酶（ＤＡＯ）、谷胱甘肽（ＧＨＳ）、肿瘤坏死因子（ＴＮＦ）和血浆内毒素（ＬＰＳ）的含量。结果表明，烫伤后１０ｈ、２ｄ、５ｄ、和８ｄ小肠ＤＡＯ水平降低，血浆ＤＡＯ升高；标准饲料（Ｃ）组血浆Ｇｌｎ在伤后２ｄ和５ｄ降低；Ｇｌｎ饲料（Ｇ）组各时点均增加，１０ｈ和８ｄ增加显著；Ｇｌｎ＋精氨酸（Ｇ＋Ａ）组在２ｄ降低。Ｃ组血浆丙氨酸、精氨酸和血氨较其它２组低。ＬＰＳ水平增高，但Ｃ与Ｇ组比较无统计学差异。病理检查见Ｃ组在伤后１０ｈ、５ｄ和８ｄ小肠绒毛萎缩变短，固有层慢性炎性细胞增多，肠壁变薄，Ｃ组和Ｇ＋Ａ组小肠粘膜厚度接近正常。提示口服Ｇｌｎ可能对烫伤大鼠小肠粘膜结构和功能有保护作用。
Changes in free amino acid(FFA),diamine oxidase(DAO),glutathione(GHS),tumor necrosis factor(TNF) and endotoxin(LtPS) level were determined in plasma and intestinaI tissue in scalded rats. Wistar rats were divided randomly into four groups:normal control(N).Early feeding with standard feed after scalding(C), early feeding with standard feed plus glutamine after scalding(G),and early feeding with standard feed plus glu- tamine and arginine after scalding(G+A).All animals(except guoup N)sustained a 30%TBSA full-thickness scald on the back and flands.Compared with normal control,the activity of intestinal DAO decreased and plasma DAO increased at l0 h,2d,5d and 8d after scald injury.In group C DAO activity was higher in plasma than that in G and G+A after scald injury.Plasma glutamine decreased at 2d and 5 d in group C,increased at l0 h in group C and decreased at 2d in group G+A. The plasma level of alanine,arginine and ammonia in group C was slightly lower than that in group G and G+A, Plasma TNF rose in C,G and G+A group animals. Plasma endotoxin in- creased,but there was no significant difference between C and G. Pathological examination of the intestinal wall revealed atrophy and shortening of intestinal villi,inflammatory cell infitration in the lamina propria,and mild edema in group C. The intestinal mucosa structure in guoup G and G+A was similar to that in normal control. The results indicate that oral glutaine may maintain intestinal mucosa function and structure after scald injury.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1996年第2期91-93,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军医药卫生"八五"重点攻关课题
