摘要
目的初步探讨烧伤后骨髓巨核细胞被噬现象的机制。方法采用光镜、电镜观察30%Ⅲ度烧伤后大鼠骨髓巨核细胞的形态变化,分别分离正常和30%Ⅲ度烧伤大鼠骨髓巨核细胞、中性粒细胞,采用Transwell双室培养法观察骨髓巨核细胞与中性粒细胞的游走关系。结果30%Ⅲ度烧伤后大鼠骨髓巨核细胞部分细胞的细胞质呈局灶样坏死,部分中性粒细胞进入到巨核细胞的细胞质进行噬食,即巨核细胞被噬现象。扫描电镜显示,烧伤后巨核细胞形态不规则,表面呈现出珊瑚样的窟窿洞,而正常组的巨核细胞呈圆形,表面光滑。分离正常和烧伤后的中性粒细胞、巨核细胞分别进行双室交叉培养,发现烧伤组大鼠骨髓巨核细胞对中性粒细胞有明显的趋化作用,而正常骨髓巨核细胞对中性粒细胞的趋化作用不明显。结论30%Ⅲ度烧伤后骨髓巨核细胞发生不同程度的损伤,且对中性粒细胞有很强的趋化作用,从而趋化后者进入损伤的巨核细胞,发生被噬现象。
Objective To observe the morphological alterations of bone marrow megakaryocytes after burns and to analyze the mechanism of megakaryocytophagia. Methods The morphological alteration of bone marrow megakaryocytes in rats after bums （30% TBSA, full thickness bum） was observed with light-microscope and electron-microscope. Megakaryocytes and neutrophils isolated from rat bone marrow （normal and burned）were co-cultivated in Transwell co-culture system to observe their transmigration relationship. Results After burns, focal necrosis took place in the cytoplasm of megakaryocytes, and megakaryocytophagia occurred when neutrophils entered into megakaryocytes and then englobed them. The results of scanning electron microscope showed the surface of megakaryocytes after burns was coral hole-like while that of normal megakaryocytes was spherical and slick. Chemotactic tests demonstrated that megakaryocytes from burned rats had strong chemotactic effect on neutrophils while normal megakaryocytes had no such effect. Conclusion Megakaryocytes are injured after burns and exert strong chemotactic effect to neutrophils, which induces neutrophils to enter the injured mgakaryocytes and thus megakaryocytophagia take place.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第20期2017-2019,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助重点项目(30230360)~~
