摘要
为研究青光眼性视网膜神经节细胞(RGCs)损害的细胞和分子机制,多种细胞培养模型已经建立,但是转基因的视网膜神经节细胞RGC-5细胞系在国内未见报道。RGC-5细胞系是应用鼠视网膜细胞通过转染技术建立的,除了其形态和电生理特性与RGCs有所不同外,细胞的生长条件以及细胞膜或者细胞内表达的物质与RGCs一致。应用RGC-5细胞系已经建立了多种凋亡模型,研究发现这些模型的细胞在凋亡时的基因变化以及对神经保护剂的反应都与RGCs一致。因此加强对RGC-5的研究和应用,对进一步阐明RGCs的损伤和保护机制将非常有意义。现就这个细胞系的建立及已做的相关研究做一综述。
Several types of culture models have been established to study the cellular and molecular mechanisms of glaucomatous optic neuropathy,but transformed retinal ganglion cells（ RGCs）, RGC-5 cell line, has not been reported in China. RGC-5 cell line can be obtained by transfer of rat retinal cells. This cell line shows positive reaction for Thy-1 ,Brn-3C,Neuritin, NMDA receptor and GABA-B receptor,and synaptophysin expression and negative for GFAP, HPC-1 ,and 8A1 ,suggesting that it represents a putative RGC clone. Comparing with RGCs, RGC-5 cell line requires the same culture condition and has a same membrane and intra-cellular expression products but different morphological and electrophysiological characteristics. In several types of apoptosis models established using RGC-5 cell line, researchers found the similar gene alteration and reaction to neuroprotectives as RGCs during the apoptosis procedure, such as nonfeminizing estrogen analogues ZYC-1, ZYC-3, ZYC-10, a semi-synthetic tetracycline derlvative-minocycline, homocysteine sigma R1-speclfic llgand （ ＋ ）-pentazoclne, upregulation of Hsp27, ect. The relevant studies about this cell line,including elementary information of transformation, transferring procedure and the details of inducing differentiation of RGC-5 cells,its morphological characteristics and culture conditions, expressing products, mitotic activity and electrophysiological properties of RGC-5 cells, some apoptosis pattern and the cell reaction to injury and various neuroprotective researches are reviewed.
出处
《眼科研究》
CSCD
北大核心
2006年第5期553-556,共4页
Chinese Ophthalmic Research
基金
国家自然科学基金资助(30460138)
