摘要
应用免疫组化Ｓ－Ｐ法检测了１１１例大肠癌及５７例癌旁粘膜组织的Ｃ－ｅｒｂＢ－２癌基因蛋白和ＰＣＮＡ表达状况。结果表明：①大肠癌组织Ｃ－ｅｒｂＢ－２癌基因蛋白和ＰＣＮＡ阳性表达率分别为４８．６％，７２．１％；癌旁粘膜仅为２８．１％，１４．８％；前者Ｃ－ｅｒｂＢ－２（Ｐ＜０．０５）ｆｏＰＣＮＡ（Ｐ＜０．０１）的表达率显著高于后者。②Ｃ－ｅｒｂＢ－２癌基因蛋白和ＰＣＮＡ阳性率与癌组织分化程度及淋巴结转移有显著相关性（Ｐ＜０．０５）。提示：测定Ｃ－ｅｒｂＢ－２癌基因产物和ＰＣＮＡ，有助于对大肠癌生物学行为的进一步认识及患者预后的分析。
munohistochemical technique(S-P method)was used to study the expressiOn of the onco-gene protein product C-erbB-2 and PCNA in serlal tissue sections of 111 cases of colorectal carci-nomas。 The results showed that the positive rates of C-erbB-2 and PCNA expression were48.6%,72.1%respectively in carcinomas and 28.1%,14.8%in the mucosa near the tissues ofcolorectal cancer.The difference between the carcinomas and the mucosa near the cancer for C-erbB-2 or PCNA staining was significant(P<0.05,P<0.01).Yet the C-erbB-2 or PCNA ex-pression was related to differentiation and metastasis of the tumors(P<0.05).Poorly differen-tiated carcinomas had higher incidence of PCNA detection,and the carcinomas with marked posi-tive C-erbB-2,PCNA expression were more likely to metastasize to lyrnph nodes。 It suggests thathigh proliferation and C-erbB-2 over-expression are common and important biological features incolorectal carcinomas and are relates to the prognosis of the tumor.
出处
《湖南医科大学学报》
CSCD
1996年第4期361-363,共3页
Bulletin of Hunan Medical University
关键词
大肠肿瘤
癌基因蛋白
PCNA
免疫组织化学
intestinal neoplasms
oncogene products
proliferating cell nuclear antigen
immunohistochernistry
