摘要
采用逆转录－ＰＣＲ的方法从骨髓基质细胞中扩增出人干细胞因子基因，并与载体ｐＫＫ２３３－２和ｐＵＣ１８重组。经该基因的限制酶酶解图谱分析及ＤＮＡ序列分析证实。
iang Dezhao(Department of Hematology, Hunan Medical University)A gene of human stem cell factor was amplified from cultured human bone marrow stromacells by reverse transcription-PCR(RT-PCR)with a pair of special primers and recombined withthe vectors pKK233-2 and PUC18.Using analysis of restriction endonuclease mapping,PCR andDNA sequencing,the cloned SCF gene was defined.The over-expression and purification of re-combinant SCF gene and its products are still in progress.
出处
《湖南医科大学学报》
CSCD
1996年第4期301-304,共4页
Bulletin of Hunan Medical University
基金
湖南省科委科研基金
关键词
基因克隆
干细胞因子
CDNA
多聚酶链反应
genes
cloning,molecular
stem cell factor
hematopoietic growth factor
reverse transcription
PCR
