摘要
用细胞培养、细胞形态学及联苯胺染色法观察中药灵芝复方对K562 白血病细胞的增殖抑制及分化诱导作用。结果显示:灵芝复方在一定浓度范围内对正常人骨髓CFUGM 生长有促进作用( P < 0 .05) ,而对白血病细胞系K562 细胞增殖表现出剂量和时间依赖性抑制( P < 0 .05) ;在诱导分化剂量下,灵芝复方可促进K562 细胞向红系分化。可见。
A series of experiments including cell culture and benzidine staining test were undertaken to investigate the effects of Ganoderma lucidum(Leyss ex Fr) Karst Compound(GLC) on the proliferation and differentiation of K562 leukemic cells. The results showed that different concentrations of GLC(from 4?mg·ml -1 to 12?mg·ml -1 ) could promote human bone marrow granulocyte macrophage colony forming unit(CFU GM) proliferation, but suppressed the growth of K562 leukemic cell colonies, and IC 50 was 9.2?mg·ml -1 . The data from liquid culture demonstrated that GLC could suppress K562 cells proliferation in a dose dependent(from 4?mg·ml -1 to 20?mg·ml -1 ?) and time dependent(from 1～5 days) manner. K562 cells could be induced to differentiate into more mature erythrocytic cells by 4?mg·ml -1 and 8?mg·ml -1 GLC. It is concluded that GLC may be a good medicine for leukemia therapy.
出处
《湖南医科大学学报》
CSCD
1999年第6期521-524,共4页
Bulletin of Hunan Medical University
基金
湖南省卫生厅基金!资助项目(编号9702)
关键词
白血病
细胞增殖
灵芝复方剂
K562细胞
Ganoderma lucidum(Leyss ex Fr) Karst
cytarabine
granulocyte macrophage colony forming unit
K562 cells
proliferation
differentiation
drug effects
