摘要
目的:寻找金银花中的抗呼吸道病毒感染的成分,并进行定量研究。方法:采用柱层析等方法分离、细胞病变法进行抗病毒作用的研究,采用 RP-HPLC 方法进行定量方法的研究。色谱系统:色谱柱为 HiQ Sil C_(18)色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(15:85),柱温:室温,检测波长240nm,流速1mL·min^(-1),进样量10μL。结果:分离得到了10种环烯醚萜苷类化合物,确认环烯醚萜苷类成分具有中等强度的抗呼吸道病毒感染的作用,并采用RP—HPLC方法测定了金银花药材中的4种环烯醚萜苷的含量。结论:环烯醚萜苷类成分为金银花中的有效成分,定量方法准确,重复性好。
Objective：To search for the antiviral components from the flowers of Lonicera japonica Thunb. and determine the contents of iridoids in Flos Lonicerae Japonicae. Methods：To isolate components with different chromatographic method and test the antiviral activities in CPE methods. The contents were determined by RP - HPLC method. The separation was carried out on a HiQ Sil C18（250 mm ×4.6 mm,5 μm） at room temperature with mobile phase of acetonitrile and water（ 15： 85）. The detection wavelength was set at 240 nm. The flow rate w, s 1.0 mL · min ^- 1. The injected volume was 10 μL. Results： 10 iridoids were isolated from Flos Lonicerae Japonicae. These iridoids showed moderate antivial activities against respiratory viral infections. The contents of 4 iridoids were determined by RP - HPLC. Condusion：The iridoids are effective components and the method is simple and accurate.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第8期1039-1042,共4页
Chinese Journal of Pharmaceutical Analysis
基金
香港 Industrial Support Fund AF/281/97
国家科技部创新药物和中药现代化资助课题
关键词
金银花
环烯醚萜苷
抗病毒
Flos Lonicerae Japonicae
iridoids
antiviral
