摘要
目的探讨白细胞介素-1(IL-1)基因多态性与尘肺易感性的关系。方法以80例Ⅰ期矽肺和45例Ⅰ期煤工尘肺为病例,以与病例年龄相差小于5岁、同性别、同民族、同一工作地点、开始接尘时间和累积接尘工龄相差不超过2年的非尘肺接尘工人为对照,进行1∶1配对。采集静脉血,用酚-氯仿法抽提DNA,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)和PCR方法检测IL-1α(-889)、IL-1β(-511)、1L-1 Ra(+2018)和IL-1Ra数目可变的串联重复序列4个基因位点的基因型,并进行单因素、多因素分析。结果两组1L-1α(-889)1/2+2/2基因型的携带率的差异有统计学意义(P＜0．01),病例组与对照组IL-1α(-889)等位基因2的频率分别为57．6%和40．8%,差异有统计学意义(P＜0．01),多因素分析表明,IL-1α(-889)突变基因型及等位基因2为尘肺的危险因素。病例组与对照组之间IL-1β(-511)1/2+2/2基因型、IL-1 Ra(+2018)1/2+2/2基因型和IL-1Ra VNTR1/2+2/ 2基因型携带率的差异均无统计学意义(P＞0．05),病例组与对照组IL-1β(-511)、IL-1 Ra(+2018)和IL-1Ra VNTR等位基因2的频率差异无统计学意义(P＞0．05)。矽肺组和煤工尘肺组间4个等位基因及基因型分布频率差异均无统计学意义(P＞0．05)。结论IL-1α(-889)基因多态性与尘肺患病有关,携带IL-1α(-889)等位基因2的接尘者患尘肺的危险性增加,未发现IL-1β(-511)、IL-1Ra (+2018)及IL-1 Ra VNTR基因多态性与尘肺发病有关联。
Objective To explore the relationship between the polymorphism of lnterleukin-1 and the pneumoconiosis susceptibility. Methods Eighty patients with silicosis and 45 with coal workers＇ pneumoconiosis （CWP） were selected while 125 male workers, Han nationality in the same workplace as the patients were selected as the controls. Between the patients and the control, the differences of age and cumulative length of service were less than five years and two years, respectively. The controls were exposed to dusts but did not suffer from pneumoconiosis. Moreover, the patients and the controls were paired by 1 ： 1. DNA was extracted from leucocytes by the hydroxybenzene chloroform method. The polymerase chain reaction restriction fragment length polymorphism （ PCR- RFLP） techniques and PCR were used to examine polymorphism of IL-1α（ - 889）, IL-1β（ - 511） and IL-1Ra （ ＋ 2018） and variable number of tandem repeats（VNTR） of 1L-1Ra. After the preliminary experiment, the most adaptive PCR reaction, the restriction enzyme digest and electrophoresis system were used. Results The difference in 1L-1α（-889） 1/2 ＋ 2/2 between the pneumoconiosis patients and the controls was significant（ P 〈 0.01 ）. The result of conditional Logistic regression showed that heterozygote and allele 2 of IL-1α（ - 889） were risk factors of pneumoconiosis. The difference in the genotype frequencies of IL-1β（ - 511 ） 1/2 ＋ 2/2, IL-1Ra（ ＋ 2018） 1/2 ＋ 2/ 2 and IL-Ra VNTR1/2 ＋ 2/2 between the patients and the controls were not statistically significant（ P 〉 0.05）. Conclusion IL-1α（ - 889） gene polymorphism is related to pneumoconiosis. Workers with IL-1α（ - 889） allele 2 are susceptible to the pneumoconiosis. The relationship between IL-1β（ - 511）, IL-1Ra（ ＋ 2018）, IL-1Ra VNTR genes polymorphisms and pneumoconiosis are not found.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第9期526-530,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家安全生产监督管理总局资助项目(2002)
关键词
尘肺
白细胞介素-1
多态性
单核苷酸
疾病易感性
Pneumoconiosis
lnterleuldn- 1
Polymorphism, Single nucleotide
Disease susceptibility
