摘要
目的观察吡咯喹啉醌(PQQ)对D-半乳糖(D-gal)致大鼠海马神经细胞衰老的影响。方法用大剂量D-gal致大鼠脑老化模型,侧脑室注射PQQ,50 d后行脑组织切片H-E染色和尼氏染色,观察海马神经元的形态学变化,流式细胞仪检测细胞的凋亡率;用TBA法和Fenton反应测海马组织自由基含量;W estern b lot观察C-FOS蛋白的表达。结果D-gal处理组鼠海马神经元胞体变小,尼氏小体的吸光度值(A)降低,细胞凋亡率和自由基含量增加,C-FOS蛋白的表达降低;而同时PQQ处理后,与对照组相比海马神经元的胞体变大,尼氏小体的A值增加,自由基含量和神经元的凋亡率无明显增加,PQQ剌激了C-FOS蛋白的表达。结论PQQ能延缓D-gal引起的大鼠海马神经细胞衰老。
Objective To investigate the effects of pyrroloquinoline quinone （PQQ） on the hippocampal neurons of aged rats induced by D-galactose（D-gal）. Methods D-gal was used to induce the model of aging rat, PQQ was administered into rat lateral intracerebroventricle. After 50 days the metamorphosis of hippocampal neurons was observed by H-E and Nissl＇ s staining. The apoptosis rate of hippocampus was tested by flow cytometry. The contents of free radical and C-FOS protem were measured. Results Compared with the control group, the size of the neurosoma was slightly changed, the optical density of Nissl＇ s was decreased, the content of free radical and the apoptosis rate increased markedly in D-gal group. After PQQ injection with D-gal, the size of neurosoma and the optical density of Nissl＇ s were markedly increased, the content of free radical and the apoptosis rate of hippocampus did not change. PQQ improved the expression of C-FOS protem. Conclusion PQQ can slow down the aging progress of hippocamal neurons induced by D-gal.
出处
《基础医学与临床》
CSCD
北大核心
2006年第9期962-966,共5页
Basic and Clinical Medicine
基金
湖北省教育厅重点项目(2003A011)
