摘要
目的:研究外源性生长激素(GH)和生长抑素(SS)对人结肠癌细胞株HT-29的影响,并探讨其作用机制。方法:人结肠癌细胞株HT-29分成正常对照组、生长抑素组(SS组)、生长激素组(GH组)和生长抑素+生长激素组(GH+SS组)。MTT法测定细胞抑制率,流式细胞仪测定细胞周期分布、增殖指数、凋亡率,RT-PCR方法测定bcl-2及baxmRNA水平。结果:生长抑素能够明显抑制人结肠癌细胞株HT-29增殖(P<0.01)、降低S期和G2/M期细胞比例(P<0.05)、降低增殖指数(PI)(P<0.05)、促进细胞凋亡(P<0.01)、降低bcl-2mRNA表达(P<0.05)、提高baxmRNA表达(P<0.01),生长激素则无明显作用。GH+SS组表现与SS组相似。结论:生长抑素可能通过抑制G0/G1期细胞进入S期和G2/M期以及促进细胞凋亡两种途径抑制体外培养的人结肠癌细胞株HT-29增殖。生长抑素可能是通过改变bax基因和bcl-2基因的表达影响肿瘤细胞的凋亡。生长激素对体外培养的人结肠癌细胞株HT-29无显著影响。
Objective To study the effects of somatostatin （SS） and growth hormone （GH） on the growth of human colonic carcinoma cell line HT-29, and its mechanism. Methods HT-29 cancer cell line was divided into 4 groups： control group, somatostatin group （SS group）, growth hormone group （GH group）, and somatostatin＋growth hormone group （SS＋ GH group）. The inhibition rates （IR） were determined by MTY assay. The cell cycle, proliferation index （PI） and apoptosis rate were determined by flow-cytometry. The mRNA expression of bax and bcl-2 was determined by RT-PCR. Results SS treatment restrained the proliferation of the cell line HT-29 （P〈0.01）, depressed the cell ratio of S and GJM phases （P〈 0.05）, depressed the PI（P〈0.05）, promoted apoptosis（P〈0.01）, depressed the bcl-2 mRNA level （P〈0.05 ）and elevated the bax mRNA level（P〈0.01 ）. There was no influence of GH on the growth of the human colonic carcinoma cell line HT- 29. The results in SS＋GH group were similar to those of the SS group. Conclusions Somatostatin could restrain the human colonic carcinoma cell line HT-29 growth by depressing cell proliferation and promoting apoptosis. The mechanism of somatostatin to promote apoptosis is probably to influnce the bax and bcl-2 mRNA expression. GH can not influence the human colonic carcinoma cell line HT-29 directly.
出处
《外科理论与实践》
2006年第5期421-424,共4页
Journal of Surgery Concepts & Practice
关键词
生长激素
生长抑素
结肠肿瘤
凋亡
Growth hormone
Somatostatin
Colonic carcinoma
Apoptosis
